杜仲提取物抗UVA致ESF-1细胞光老化作用的机制研究

目的:探讨杜仲提取物(EE)抗UVA致人真皮成纤维细胞光老化的作用机制。方法:将体外培养的ESF-1细胞通过UVA照射建立细胞光老化模型,给予500μg/mL、250μg/mL、125μg/mL、62.5μg/mL 4种不同浓度的杜仲提取物,采用ELISA法检测细胞培养液中Ⅰ型胶原蛋白(ColⅠ)、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)的含量。结果:ESF-1细胞接受40J/cm的UVA照射后,细胞培养液中MMP-1、MMP-3含量明显升高(P0.05),ColⅠ含量明显降低(P0.05);给予EE后,细胞培养液中ColⅠ含量明显升高(P0.05),MMP-1含量明显降低(P0.05),MMP-3含量无明显变化。结论:杜仲提取物能抑制UVA引起的人MMP-1及MMP-3的异常分泌,降低ColⅠ的降解。

Study on Function Mechanism of Eucommia Extract against Photoaging of ESF- 1 Cells induced by UVA

Objective ： To investigate the mechanism of Eucommia extract against photoaging of human fibroblasts induced by UVA radiation. Methods： Cultured normal skin fibroblast was irradiated with UVA to develop pho-toaging model and treated with different dose of Eucommia extract. The amount of MMP -1 ,MMP- 3 ,Col I was detected by ELISA. Results： Cultured normal skin fibroblast irradiated by 40J. cm-2, the amount of MMP - 1, MMP - 3 increased obviously （ P 〈0. 05 ）, Col I decreased obviously （ P 〈 0. 05 ） ; after provided with Eucommia extract, the amount of MMP - 1 decreased obviously （ P 〈 0. 05 ）, the amount of Col I increased obviously（P 〈0. 05 ） and MMP- 3 was not obvious. Conclusions： Eucommia extract could inhibite the level of MMP - 1 and MMP -3 and reduce the degradation of Col I that induced by UVA.