碱性成纤维细胞生长因子及其受体在肾癌组织中的表达

碱性成纤维细胞生长因子（ｂＦＧＦ）可诱发血管形成、刺激癌细胞生长。本研究试图通过检测ｂＦＧＦ及其受体ＦＧＦＲ－１在正常肾和肾癌组织的表达方式，探讨其临床意义。作者针对３６例石蜡包埋的肾癌及其正常肾组织，配合微波炉抗原修复法进行ｂＦＧＦ和ＦＧＦＲ－１的ＬＳＡＢ免疫组化染色。结果显示：ｂＦＧＦ与ＦＧＦＲ－１的染色结果基本一致；全部正常肾组织和２９例肾癌的细胞外基质为非均一性阳性染色；１２例原发性肾癌、２例癌转移灶和体外培养的ＧＲＣ－１细胞的癌细胞胞浆为阳性染色，且多为均一性染色。癌细胞胞浆的ｂＦＧＦ染色多见于Ｔ３－４期肿瘤，与Ｔ１－２期比较有显著性差异（Ｐ＝０．０２）。作者认为，ｂＦＧＦ和ＦＧＦＲ－１可同时表达于正常肾和肾癌组织，提示ｂＦＧＦ和ＦＧＦＲ－１的表达与功能密切相关；癌细胞胞浆ｂＦＧＦ阳性表达与肿瘤分期有关，提示ｂＦＧＦ是促进肾癌发展的原因之一。

Expressionofbasicfibroblastgrowthfactoranditsreceptorinrenalcelcarcinoma

Basicfibroblastgrowthfactor(bFGF)isapluripotentpolypeptidewhichplaysanim-portantroleintumorprogresionandangiogensis.Wedeterminedtheexpressionandlocalizationpat-ternsofbFGFandoneofitsreceptor(FGFR-1)innomalrenalaswelasinrenalcancers.Theresultswerecomparedwithclinicopathologicfeatures.UsingbFGFandFGFR-1antiboty,therepairingmethodofantigenwithmicrowaveovenheatingandLSABimmunohistochemistryweusedin36casesofparaf-finembeddedrenalcelcarcinoma(RCC)andtheirpairednormalrenaltissues.TheexpressionofbFGFandFGFR-1wasnearlyconsistent.ThenormalrenaltissuesandECMof29casesofrenalcancertisuesshowedheterogenousimmunoreactivities.Renalcancercelcytoplasmof12primarytumorsand2metastatictumors,asinthecytoplasmicbFGFofculturedGRC-1cels,werepositivelyhomogenousstained.ThebFGFandFGFR-1canbeconsistentlyexpressedinnormalrenalandrenalcancertissues,reflectingthattheexpressionandfunctionofthesesubstanceswerecloselyasociated.ThecytoplasmicbFGFexpressionofrenalcancerwasrelatedtotumorstages,suggestingthatbFGFplaysanimportantroleintheprogressionofrenalcelcarcinoma.