香椿子多酚对糖尿病大鼠早期肝损伤保护作用

目的:观察香椿子多酚(TSTP)对链脲佐菌素(STZ)诱导的大鼠糖尿病早期肝损伤的保护作用。方法:一次性ip链脲佐菌素(55 mg·kg-1),造模72 h后禁食不禁水,用血糖仪检测,空腹血糖大于11.1 mmol·L-1为造模成功大鼠。将造模成功大鼠随机分为模型组、香椿子多酚高、低剂量组(75,45 mg·kg-1)、格列本脲组(10 mg·kg-1),另取8只作为正常组,每天ig1次,连续14 d。第15 d处死后取其血清和肝脏。血清测空腹血糖(FBG),谷丙转氨酶(ALT),谷草转氨酶(AST);取肝脏测定脏器指数,肝脏石蜡切片,做HE染色。结果:香椿子多酚能降低大鼠空腹血糖,高、低剂量组7 d血糖分别为(11.79±2.75),(12.55±3.35)mmol·L-1,与模型组(15.53±2.33)mmol·L-1比较血糖降低(P<0.05),14 d血糖分别为(10.18±2.34),(11.80±2.74)mmol·L-1,与模型组(15.57±2.69)mmol·L-1比较差异有显著性(P<0.01);不能降低血清ALT及AST含量;香椿子多酚高剂量组大鼠肝脏脏器指数为(3.616±0.358),与模型组比较显著降低(P<0.05),改善大鼠肝脏形态。结论:香椿子多酚对糖尿病大鼠早期肝脏损伤具有一定的保护作用。

Protective Effect of Total Polyphenols from Seeds of Toona sinensis on Hepatic Injury at Early Stage in Diabetic Rats

Objective: To study protective effects of total polyphenols from the seeds of Toona sinensis (TSTP)on hepatic injury at early stage in diabetic rats. Method: Diabetic models were induced by once intraperitoneal injection of STZ (55 mg ·kg^-1).Then the blood glucose was detected after 72 hours. when hypoglycemic point>11.1 mmol ·L^-1 the rats model were successful. Diabetic rats were randomly divided into model group,glibenclamide group(10 mg ·kg^-1) and high-(75 mg ·kg^-1) and low(45 mg ·kg^-1)-doses of TSTP groups. At the same time,8 rats were choosen as normal group. All groups were fed qd for 14 consecutive. At fifteen day, all rats were anaesthetized and then were killed to remove Hepars and collect the blood sample. The fasting blood glucose(FBG), AST,ALT were detected. the liver index was calculated. The pathological changes of the liver were evaluated with HE. Result: TSTP can decrease FBG. High-and low-doses of TSTP groups FBG at 7 days were (11.79±2.75),(12.55±3.35) mmol ·L^-1and at 14 day were (10.18±2.34),(11.80±2.74) mmol ·L^-1,Compared to modles,the FBG decreased(P< 0.05,P< 0.01);ALT and AST did not decrease. High dose group decrease liver index(3.616±0.358 3),improve pathological morphous. Conclusion: TSTP has a protective effect on liver injury at early stage in diabetic rats.