全反式维甲酸对高糖诱导HK-2细胞FN、LN表达的影响

目的观察全反式维甲酸（ATRA）对高糖诱导人肾小管上皮细胞（HK-2）纤维连接蛋白（FN）、层粘连蛋白（LN）表达的影响,探讨ATRA延缓糖尿病肾病肾间质纤维化的发生机制。方法体外培养的HK-2细胞随机分为正常对照组（NG组,含5.5mmol/LD-glucose）;高糖组（HG组,含30mmol/LD-glucose）;渗透压对照组（MG组,含5.5mmol/LD-glucose＋24.5mmol/Lmannitol）;全反式维甲酸低浓度组（ATRA1组,HG＋10^-7mol/LATRA）;全反式维甲酸中浓度组（ATRA2组,HG＋10^-6mol/LATRA）;全反式维甲酸高浓度组（ATRA3组,HG＋10^-5mol/LATRA）;药物溶剂无水乙醇组（HG＋10^-2mol/L无水乙醇）;卡托普利组（HG＋10^-5mol/Lcaptopril）。各组细胞分别培养24h、48h和72h后收集细胞上清液。采用酶联免疫吸附法（ELISA）检测培养细胞上清液FN、LN的浓度。结果高糖可增加HK-2细胞培养上清的FN、LN的水平（P〈0.01）,ATRA可以部分抑制高糖的作用（P〈0.01）。结论高糖可以促进体外培养的HK-2细胞FN、LN的表达,ATRA可以抑制高糖的作用,这可能是ATRA延缓糖尿病肾病肾间质纤维化进展的机制之一。

The Effects of All-trans Retinoic Acid on the Expression of Fibronectin and Laminin in Renal Tubular Epithelial Cells

Objective To investigate the effects of all-trans retinoic acid on the expression of Fibronectin(FN) and Laminin(LN) in human renal tubular epithelial cells(HK-2) so as to explore the role of all-trans retinoic acid on the pathogenesis of delaying renal interstitial fibrosis.Methods HK-2 cells cultured in vitro were divided randomly into eight groups:normal glucose group with 5.5 mM D-glucose(NG);high glucose group with 30 mM D-glucose(HG);mannitol group with 5.5 mM D-glucose and 24.5 mM mannitol(MG);all tr...