糖代谢异常的代谢综合征大鼠模型的建立及评价

目的:建立糖代谢异常的代谢综合征大鼠模型及其评价标准。方法:采用高脂高盐高糖饮食配合饮用蔗糖水喂养Wistar雄性大鼠12周,喂养结束后检测其体重、血压、血糖、甘油三酯、高密度脂蛋白及胰岛素水平。根据中华医学会糖尿病学分会关于代谢综合征(MS)的诊断标准和统计学原理建立模型评价标准。结果:第8周开始,高脂高糖高盐膳食组大鼠体重(367±17)g和收缩压(130.67±4.31)mmHg与普通膳食对照组比较显著升高(P<0.01);第12周时,大鼠空腹血糖(10.51±0.89)mmol·L-1、甘油三酯(1.63±0.23)mmol·L-1、胰岛素水平(1.97±1.07)mmol·L-1显著升高(P<0.01或P<0.05),高密度脂蛋白(0.54±0.07)mmol·L-1显著降低(P<0.01)。根据评价标准,模型成功率达到80%。结论:实验成功建立了糖代谢异常的MS大鼠模型,并初步建立了该模型的评价标准。

Establishment and Evaluation of Rat Model of Metabolic Syndrome with Abnormal Glucose Metabolism

Objective: To establish and to evaluate rat model of metabolic syndrome (MS) with abnormal glucose metablism. Method: Wistar male rats were fed with a diet of high fat, salt and sugar for 12 weeks. Afterwards, body weight, blood pressure, blood glucose, triglycerides, high density lipoprotein and insulin level were determined. According to the MS diagnostic criteria established by Chinese Diabetes Society (CDS) and statistical theory, the model was evaluated. Result: In the first 8 weeks, rats were significantly increased body weight and systolic blood pressure; in the first 12 weeks, fasting blood glucose, triglycerides, insulin level were significantly elevated, high-density lipoprotein significantly reduced. Based on the evaluation criteria, a success rate of 80% for the model was found. Conclusion: We successfully established the rat model of MS with abnormal glucose metabolism, and also the preliminary evaluation criteria for the model.