吴茱萸药材薄层色谱指纹图谱研究

目的:建立吴茱萸药材薄层色谱指纹图谱,用于产地、真伪鉴别及品质控制。方法:采用薄层色谱法对12批贵州余庆吴茱萸药材、9批其他不同产地吴茱萸药材及2种伪品进行薄层色谱的比较分析。结果:贵州余庆产12批吴茱萸药材的薄层色谱图和轮廓图十分相似,均有6个斑点和对应的6个峰;各峰在峰强度上有差异,体现了不同产地吴茱萸所含化学成分在含量上有差别;不同产地吴茱萸的主成分组成基本相同,但各组分的含量有较大的差异;2种伪品没有上述特征。结论:方法简单易于操作,便于推广应用,可用于采购及生产中的快速检验,为评价吴茱萸药材品质优劣和GAP规范化种植、种植标准操作规程的制定以及质量监控提供科学依据。

Fingerprint Analysis of Evodia rutaecarpa by TLC

Objective: To establish TLC fingerprint of Evodia rutaecarpa for the place of production,the authenticity of identification and quality control. Method: TLC was used to analyze comparatively 12 groups of crude drugs E. rutaecarpa from Guizhou Yuqing,9 groups of crude drugs E. rutaecarpa from other different places of production and 2 Counterfeits. Result: The TLC chromatogram and the contour chromatogram of 12 groups of crude drugs from Guizhou Yuqing were very similar,which had 6 spots and 6 corresponding peaks. The intensity of each peak was different,reflecting that the content of chemical ingredients contained in E.rutaecarpa from different places were different.The main kinds of ingredient in E.rutaecarpa from different places were nearly the same,but the content of each component was different. The two kinds of counterfeits had no such characteristics. Conclusion: The method was simple and easy to operate,which can be used widely,such as rapid tests in procurement and production. And it provided a scientific basis to judge the quality of E.rutaecarpa and standardized planting of GAP,as well as to draw up the standard operating procedures of planting and the quality control of E.rutaecarpa.