山茱萸水提取物对黑素瘤细胞与角质形成细胞共培养模型黑素合成的影响

目的:考察山茱萸水提取物对体外构建的人黑素瘤细胞(A375)与角质形成细胞(Hacat)单层混合共培养模型黑素合成的影响。方法:将山茱萸水提取物制备成含生药0.75～12 g.L-15个质量浓度,细胞给药,通过MTT法、NaOH裂解法、多巴氧化法分别测定给药前后A375细胞与Hacat细胞共培养模型的细胞活力、黑素含量和酪氨酸酶活性。结果:与空白对照组相比,6,3,1.5,0.75 g.L-1山茱萸水提取物对共培养体系的细胞增殖率影响不大,对共培养体系黑素生成均有显著差异(P<0.01),对共培养体系酪氨酸酶活性有差异(P<0.05或P<0.01);与阳性对照药物熊果苷相比,6,3 g.L-1山茱萸水提取物对共培养体系黑素生成有显著差异(P<0.01),作用强于熊果苷,1.5,0.75 g.L-1山茱萸水提取物对共培养体系黑素生成无差异;与阳性对照药物熊果苷相比,6,3,1.5,0.75 g.L-1山茱萸水提取物对共培养体系酪氨酸酶活性无差异。结论:山茱萸水提取物能够通过抑制酪氨酸酶活性来抑制A375细胞和Hacat细胞共培养体系的黑素合成,为临床使用山茱萸治疗黄褐斑提供了实验依据。

Effects of Water Extract of Corni Fructus on Melanin Synthesis in Co-culture System of Human Melanocytes and Kertinocytes

Objective: To evaluate the effects of water extract of Corni Fructus on the melanin synthesis in the co-culture system of human melanocytes and kertinocytes. Method: The proliferation,the tyrosinase activity and melanin contents of A375 and Hacat co-culture cells were measured by MTT,dopaoxidase and NaOH methods,respectively. Result: The tyrosinase activity and melanin content were decreased significantly (P<0.05) by the water extract of Corni Fructus. Dose dependence of the water extract was observed from 0.75 -12 g ·L^-1. But the cell proliferation was not inhibited markedly (P<0.01). Conclusion: The melanin synthesis of A375 and Hacat co-culture cell system was decreased significantly by water extract of Corni Fructus. And tyrosinase activity inhibition was proved to be the mechanism of melanin decrease.