复心煎抗大鼠心肌缺血再灌注损伤机制研究

目的:探讨复心煎对大鼠心肌缺血再灌注损伤保护作用的机制。方法:实验大鼠随机分为伪手术组、模型组、氟伐他汀组(4 mg·kg-1)及复心煎高、低剂量组(按生药量计为28,14 g·kg-1),预先ig给药1周后,采用结扎冠状动脉法制备心肌缺血再灌注损伤模型,最后处死大鼠,HE染色观察大鼠心肌组织病理形态学变化,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及一氧化氮(NO)的含量,TUNEL法测定心肌细胞的凋亡,RT-PCR测定心肌组织诱导型一氧化氮合酶iNOS mRNA的表达。结果:复心煎高、低剂量组能明显改善缺血再灌注所致心肌组织病理损害;复心煎高剂量组MDA含量(4.49±0.42)μmol·L-1,与模型组(5.27±0.69)μmol·L-1比较明显降低(P<0.05);高剂量组SOD和GSH-Px活性分别为(127.64±19.54)U·mL-1、(534.10±40.82)μmol·L-1,与模型组(104.58±20.54)U·mL-1、(483.85±33.29)μmol·L-1比较明显升高(P<0.05);复心煎低剂量组与模型组相比,各血清指标无显著性差异;复心煎高、低剂量组凋亡指数分别为(19.11±5.78)%,(24.63±4.10)%,与模型组(29.94±6.77)%比较明显降低(P<0.01,P<0.05);复心煎能一定程度上抑制iNOS mRNA的表达。结论:复心煎对大鼠心肌缺血再灌注损伤具有保护作用,其机制可能与清除氧自由基,抑制心肌细胞凋亡和降低iNOS mRNA表达有关。

Mechanisms of Fuxin Jian against Myocardial Ischemia Reperfusion Injury in Rats

Objective: To study the mechanisms of Fuxin Jian against myocardial ischemia reperfusion injury in rats. Method: The experimental animals were randomly divided into the sham operation group,ischemia reperfusion model group,fluvastatin group(4 mg ·kg^-1),high dose of Fuxin Jian group(28 g ·kg^-1 )and low dose of Fuxin Jian group(14 g ·kg^-1).The rats in each group were administrated intragastrically with defined preventive drugs for seven days.The myocardial ischemia reperfusion injury model was established by ligating left anterior descending coronary.All rats were dissected to observe pathological changes of cardiac muscle by HE staining.The contents of superoxide dismutase(SOD), malonaldenhyde(MDA), glutathione peroxidase(GSH-Px) and nitric oxide(NO) in serum were measured.Apoptosis in myocardial tissue was detected by TUNEL.The expression of inducible nitric oxide synthase(iNOS)mRNA in rat myocardium was detected by RT-PCR. Result: Fuxin Jian could obviously ameliorate the pathological lesion caused by ischemia reperfusion injury.Compared with model group,the contents of MDA in high dose group of Fuxin Jian were decreased(P<0.05),and the activities of SOD and GSH-Px were increased(P<0.05).But there was no significant difference between low dose group and model group.The number of apoptotic cells were reduced significantly in both groups of Fuxin Jian(P<0.01 or P<0.05)and the expression of iNOS mRNA was inhibited to some extent. Conclusion: Fuxin Jian has protective effects on myocardial ischemia reperfusion in rats.The mechanisms might be related to scavenging oxygen free radicals,inhibiting apoptosis and reducing the expression of iNOS mRNA.