| 血管内皮细胞对成纤维细胞增殖活性的影响 |
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| 引用本文： | 阎国富,何威,刘荣卿.血管内皮细胞对成纤维细胞增殖活性的影响[J].中华皮肤科杂志,2000,33(1):29-31. |
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| 作者姓名： | 阎国富 何威 刘荣卿 |
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| 作者单位： | 重庆第三军医大学新桥医院皮肤科(阎国富!400037，何威!400037，何云志!400037，张国威 !400037)，西南医院皮肤科(刘荣卿) |
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| 基金项目： | 国家自然科学基金资助课题!（39600154） |
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| 摘 要： | 目的 观察内皮细胞对皮肤成纤维细胞增殖活性的影响。方法 对培养脐静脉内皮细胞，收集内皮细胞条件培养，利用＾３Ｈ－ＴｄＲ掺入的方法检测内皮细胞条件培养液对正常人真皮成纤维细胞ＤＮＡ合成的影响，利用流式细胞仪分析内皮细胞条件培养液对正常人真皮成纤维细胞细胞周期的影响。结果 脐静脉内皮细胞条件培养液能显著增加皮肤成纤维细胞ＤＮＡ合成，细胞周期分析显示，Ｓ期成纤维细胞的比例显著增加。结论 内皮细胞通过分泌
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| 关 键 词： | 成纤维细胞 DNA合成 血管内皮细胞 真皮纤维化 |
Effect of Vascular Endothelial Cells on Fibroblast Proliferation |
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| YAN Guofu,HE Wei,LIU Rongqing,et al..Effect of Vascular Endothelial Cells on Fibroblast Proliferation[J].Chinese Journal of Dermatology,2000,33(1):29-31. |
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| Authors: | YAN Guofu HE Wei LIU Rongqing |
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| Institution: | YAN Guofu,HE Wei,LIU Rongqing,et al. Department of Dermatology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037 |
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| Abstract: | Objective To investigate the effects of vascular endothelial cells on the proliferation of fibroblasts. Methods The interaction between endothelial cell and fibroblast interactions in vitro was explored by investigating the modulation of fibroblast properties by endothelial cell conditioned medium (EC CM) in microwell fibroblast cultures. 3H TdR incorporation and flow cytometry analysis was used for assessing the fibroblast proliferation. Results EC CM stimulated DNA synthesis of fibroblast. The ratio of fibroblasts in S cycle was significantly increased. Conclusion The endothelial cells could affect the biological characteristics of fibroblasts. |
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| Keywords: | Fibroblasts DNA synthesis Endothelial cells |
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