北京地区育龄妇女泌尿生殖道沙眼衣原体感染状况及基因分型初步研究

目的对北京地区育龄妇女沙眼衣原体的感染状况及基因分型进行初步研究。方法利用胶体金免疫法对 177例计划生育门诊及妇科体检的育龄妇女进行筛查，对 9例沙眼衣原体感染阳性者的拭子标本直接进行套式 PCR扩增主要外膜蛋白（ MOMP）基因。将扩增的 DNA进行限制性内切酶长度多态性分析，并对 MOMP基因易变区 VS1、 VS2、 VS3、 VS4进行基因序列分析。结果 177名育龄妇女有 9例沙眼衣原体感染阳性， 9份标本均扩增出 1.1 kb目的 DNA，经限制性内切酶长度多态性分析， 4例确定为 F型， 3例为 E型， 2例为 D型。利用 373A自动序列分析仪，对 MOMP基因易变区 VS1、 VS2、 VS3、 VS4进行基因序列分析，与标准序列比较发现， 2例为 D型， 3例为 E型， 4例为 F型，与限制性内切酶长度多态性分析结果相同，但其中 4例有 MOMP基因变异存在。结论北京地区健康育龄妇女存在一定比例的沙眼衣原体感染，利用对 MOMP基因的多态性分析可以对沙眼衣原体的流行情况作出分析。

Study of Chlamydial Urogenital Infection and MOMP Gene Variants in Child- bearing Women in Beijing

Objective To investigate Chlamydia trachomatis infection and gene variants in child- bearing women in Beijing. Methods Endocervical samples were collected from 177 patients in a family planning clinic and a gynecologic clinic. C.trachomatis infection was screened with a fast diagnostic kit .Major outer membrane protein(MOMP) gene was amplified by a nested PCR . A 1.1kb amplified product was obtained and studied by restriction fragment length polymorphism analysis(RFLP). Variant domains of MOMP gene VS1,VS2,VS3,VS4 were sequenced with 373A automated sequencing system. Results C. trachomatis was found in 9 of 177 samples. Four samples were identified as F genotype, 3 E genotype and 2 D genotype. Variant domains of MOMP gene, VS1, VS2, VS3, VS4 were sequenced and the results were consistent with those of RFLP, however, mutations were found in MOMP gene in 4 samples. Conclusion C.trachomatis urogenital infection has been identified in a portion of child- bearing women in Beijing. C.trachomatis can be well genotyped with RFLP or gene sequencing of MOMP gene.