银屑病患者皮损中细胞凋亡情况的评价

目的 探讨细胞恨与银屑病的关系。方法 用免疫组化ＳＰ法检测银屑病皮损与非皮损中Ｂａｘ、Ｆａｓ、Ｆａｓ－１的表达，并用ＴＵＮＥＬ（末端脱氧核苷酰转移介导的ｄ－ＵＴＰ－生物素缺口本端标记）技术检测细胞凋亡情况。结果 与正常人相比，银屑病表皮角质形成细胞，真皮炎症细胞及炎症细胞及血管内皮均存在不同程度的上述蛋白的过度表达，伴细胞凋亡增多，部分在治疗后末完全恢复正常。结论 推测银屑病皮损中角质形成对细胞过

Evaluation of Cell Apoptosis in Lesional and Non- lesional Psoriatic Skin

Objective To study the relationship between cell apoptosis and psoriasis. Methods The expression of Bax, Fas and Fas- L was studied with immunohistochemical technique(SP), and apoptosis was detected with terminal deoxynucleotidyl transferase- mediated dUTP- biotin nick end labeling (TUNEL) assay in lesional and non- lesional psoriatic skin.Results In comparison with normal skin, there was an over- expression of Bax, Fas and Fas- L in epidermal keratinocytes, inflammatory cells and vascular endothelium in psoriatic skin, and accompanied with up- regulation of apoptosis. The above abnormalities were not corrected completely after effective treatment in some cases. Conclusions The increase of apoptotic keratinocytes is possibly one kind of internal homeostatic mechanism against the hyperplasia of epidermal cells, and might relate to abnormal expression of apoptosis- associated proteins Bax, Fas and Fas- L. The Fas system might also take part in unusual local immunoreaction in psoriasis.