| 瘢痕疙瘩组织中内皮素－1及碱性成纤维细胞生长因子mRNA的表达 |
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| 引用本文： | 阎国富,何威,刘荣卿.瘢痕疙瘩组织中内皮素－1及碱性成纤维细胞生长因子mRNA的表达[J].中华皮肤科杂志,2000,33(4). |
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| 作者姓名： | 阎国富 何威 刘荣卿 |
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| 作者单位： | 第三军医大学附属新桥医院皮肤科 !400037重庆，第三军医大学附属新桥医院皮肤科 !400037重庆，第三军医大学附属西南医院皮肤科 |
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| 基金项目： | 国家自然科学基金资助课题! (39600154) |
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| 摘 要： | 目的 了解内皮素-1和碱性成纤维细胞生长因子 (bFGF)在瘢痕疙瘩组织的表达情况。 方法 组织活检标本分为 3组，瘢痕疙瘩、萎缩性瘢痕和正常皮肤。用地高辛标记的 cDNA探针，采取冰冻切片原位杂交的方法检测内皮素-1和 bFGF mRNA的表达。结果 瘢痕疙瘩真皮组织内内皮素-1 mRNA的表达明显强于萎缩性瘢痕和对照组。阳性染色主要位于真皮浅层血管和部分真皮成纤维细胞。瘢痕疙瘩组 6/8例真皮成纤维细胞内皮素-1 mRNA阳性，且呈弥漫性分布；萎缩性瘢痕和正常皮肤真皮成纤维细胞内皮素-1 mRNA无阳性着色。结论 内皮素-1和 bFGF mRNA在瘢痕疙瘩组织中的过度表达提示内皮素-1和 bFGF可能在瘢痕疙瘩的形成中起一定作用。
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| 关 键 词： | 瘢痕疙瘩 内皮缩血管肽-1 成纤维细胞生长因子 碱性 |
Expression of Endothelin-1 and bFGF mRNA in Keloid Tissues |
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| YAN Guofu,HE Wei,LIU Rongqing.Expression of Endothelin-1 and bFGF mRNA in Keloid Tissues[J].Chinese Journal of Dermatology,2000,33(4). |
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| Authors: | YAN Guofu HE Wei LIU Rongqing |
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| Institution: | YAN Guofu,HE Wei,LIU Rongqing.Department of Dermatology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037 |
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| Abstract: | Objective To determine the expression of endothelin-1(ET-1) and basic fibroblast growth factor(bFGF) mRNA in keloid lesion. Methods Skin biopsies were obtained from patients with keloid, normal scar and healthy controls. The expression of ET-1 and bFGF mRNA was evaluated by in situ hybridization with Dig labelled cDNA probe. Results Dermal ET-1 and bFGF mRNA staining was more intensive in keloid lesion than that in scar and control tissue. Increased ET-1 staining was mainly observed in the superficial dermal vessels and dermal fibroblasts. ET-1 positive fibroblasts were found in 6 of 8 patients with keloid and diffusely distributed. Conclusion The overexpression of ET-1 and bFGF in keloid indicates that ET-1 and bFGF may play a role in the formation of keloid. |
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| Keywords: | Keloid Endothelin-1 Fibroblast growth factor basic |
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