新城鸡瘟病毒诱导人粘附性单个核细胞产生一氧化氮与细胞毒性

本文采用Griess试剂、间接免疫荧光法和同位素释放等方法,发现新城鸡瘟病毒Losota系（NDV-L）与人外周血粘附性单个核细胞（a-MCs）作用2～4h后,可稳定地吸附在a-PBMCs表面,并使其释放一氧化氮（NO）,释放量与阳性对照组（BCG-LPS作用的a-PBMCs）相近;采用~3H-TdR释放法测定NDV-L作用的a-PBMCs对K_(562)靶细胞的细胞毒活性,发现具有明显的杀伤效应,且该杀伤效应与NO的产生有一定的依赖性。

Study on the Cytotoxic Activity and Nitric Oxide (NO) Production of Adherent Peripheral Blood Mononuclear Cells (PBMC) Infected with NDV Losota Strain

In this paper, the relationship between cytotoxic activity and NO production of the adherent PBMC infected with Newcastle disease virus (NDV) Losota strain (NDV-L) was investigated. It was found that NDV-L could adsorb steady on the surface of adherent PBMC and cause the release of NO from the cells 2-4 h after infection. The amount of NO release approached somewhat to that of the positive control group (BCG-LPS-adherent PBMC) .Meanwhile, a significant cytotoxic activity to the K562 target cells was demonstrated by means of the 3H-TdR release technique and a correlation existed between the cytotoxic effect and the production of NO.