| GM—CSF促进乙肝病毒基因疫苗诱导的抗体产生 |
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| 引用本文： | 张彤 陈宗德. GM—CSF促进乙肝病毒基因疫苗诱导的抗体产生[J]. 现代免疫学, 1998, 18(2): 90-93 |
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| 作者姓名： | 张彤 陈宗德 |
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| 作者单位： | 河南医科大学微生物学与免疫学教研室!郑州450052 |
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| 摘 要： | 将构建的编码乙肝病毒PreS2+S蛋白的真核表达质粒注射于C57BL/6小鼠胫前肌内,3d后,在同一部位注射rhGM-CSF。基因疫苗接种2周后,血清中可测到抗-HBs,两月后,抗体水平达到高峰,并保持高水平至少2月以上;肌内注射GM-CSF可提高血清抗-HBs水平,提示GM-CSF可作为基因疫苗的佐剂。
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| 关 键 词： | GM-CSF 乙肝病毒 基因疫苗 抗-HBs |
Ehancement of GM-CSF on Production of Anti-HBsAg in Mice Induced by HBV Genetic Vaccine |
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| Zhang Tong,Chen Zongde,Zhang Guanglei,et al.. Ehancement of GM-CSF on Production of Anti-HBsAg in Mice Induced by HBV Genetic Vaccine[J]. Current Immunology, 1998, 18(2): 90-93 |
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| Authors: | Zhang Tong Chen Zongde Zhang Guanglei et al. |
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| Abstract: | The eukaryotic expression plasmid encoding HBV envelop PreS2+S protein was injected into tibialis anterior muscles of C57BL/6 mice. After 3 days of genetic vaccine inoculation, re-combinant rhGM-CSF was administered at the same sites. ELISA was used to detect the anti-HBs in mice sera at various times after gene transfer. It was found that anti-HBs was detected in sera of mice (≥10mIU/ml) 2 weeks after plasmids injection. The level of antibody attained the peak two months after genetic vaccination and kept in high titer for at least 2 months ; rhGM-CSF injected intramuscularly could significantly (P<0. 01) promote the production of anti-HBs induced by HBV DNA vaccine. The results suggested that GM-CSF can act as an immunological adjuvant for genetic vaccines. |
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| Keywords: | GM-CSF HBV genetic vaccine anti-HBs |
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