慢性HCV感染状态与机体细胞免疫功能关系的探讨

目的:探讨慢性丙型肝炎病毒(HCV)感染患者丙型肝炎病毒RNA(HCV RNA)增殖状态与T细胞亚群功能及血浆中IL-2和slL-2R活性的关系。方法:以间接免疫荧光法,ELISA法和RT-PCR分别检测75名慢性HCV感染患者(其中HCV RNA阳性47例,HCV RNA阴性28例)外周血T细胞亚群,IL-2及sIL-2R的水平和HCV RNA。结果:慢性HCV感染患者周围血CD3~+,CD4~+淋巴细胞亚群,CD4~+/CD8~+比值及IL-2水平均显著低于正常对照组(P<0.01),而sIL-2R明显升高(P<0.01);血清HCV RNA阳性患者T细胞亚群,CD4~+/CD8~+比值及IL-2水平显著低于HCV RNA阴性患者(p<0.01)。结论:慢性HCV感染患者的机体免疫功能紊乱。细胞免疫功能低下,HCV RNA阳性患者较阴性患者更甚,提示细胞免疫功能受抑可能是HCV持续增殖的原因。

Study on the Relationship between Immune Function and the Chronic HCV Infection

To investigate the relationship between cellular immune function and the infection status of viruses in chronic HCV infection,peripheral blood T-lymphocyte subsets and serum levels of IL-2 and sIL-2R were determined by indirect immuno-fluorescence and ELISA in 75 patients with chronic HCV infection, of which 45 cases were HCV-RNA positive and 28 cases HCV-RNA negative. The HCV-RNA was determined by RT-PCR. It was found that the CD3 and CD4 T cell subsets , CD4 /CD8 ratio and the blood levels of IL-2 of patients were significantly lower than those of the controls, but the level of sIL-2 increased significantly. The T cell subsets, CD4 /CD8 ratio and blood levels of IL-2 of HCV-RNA positive cases were significantly lower than those of the HCV-RNA negative cases. It suggests that suppression of the cell mediated immunity might be the cause of HCV persistant infection.