宫颈癌HeLa细胞摄取ACM-LDL及游离ACM的比较研究

目的比较宫颈癌HeLa细胞对阿克拉霉素-低密度脂蛋白复合物(ACM-LDI)及游离ACM的摄取量及ACM-LDL对HeLa细胞DNA及RNA合成的抑制作用.方法采用保温交换法制备ACM-LDL复合物,观察在相同的作用时间下,HeLa细胞对ACM-LDL及游离ACM的摄取量,应用流式细胞仪测定HeLa细胞内DNA、RNA的含量.结果①相同时间内,宫颈癌HeLa细胞摄取ACM-LDL复合物数量显著高于ACM;②细胞对复合物的摄取量可因天然LDL的竞争抑制而减少;③细胞对复合物的摄取受LDL受体(LDLR)饱和度的限制;④复合物对HeLa细胞DNA、RNA的合成具有较强的抑制作用.结论LDI与ACM结合,对LDL和ACM的结构、理化性质及代谢特征均无影响,以LDI为化疗药物载体,可提高宫颈癌细胞内的药物含量,增加药物的抑癌效果.

COMPARISON OF INTAKES OF ACM-LDL COMPLEX AND FREE ACM BY HELA CELLS OF UTERINE CERVICAL CARCINOMA

Objective:To study cellular intake of aclacinomycin LDL(ACM LDL) complex and free ACM by HeLa cells of uterine carcinoma.Inhibition effect of this complex on the synthesis of DNA and RNA of the cancer cells was also studied.Methods:ACM LDL complex was prepared by incubation cross over method.Intake of the complex by HeLa cells was compared in the same time interval.Flowcytometry was used to syudy the inhibition effect of DNA and RNA by the complex.Results:Cellular intake of ACM LDL was siginificantly higher than that of free ACM during the same time interval.Native LDL could compete with ACM LDL complex thus lowering cellular intake of the complex.Intake of the complex was constricted by the saturation of LDL receptors.ACM LDL complex had stronger inhibition effect on the synthesis of DNA and RNA of the cancer cells.Conclusion:The structure,physiochemical quality,and metabolic characteristics are not affected after LDL binds to ACM.As a drug carrier,LDL can enhance the intracellular amount of ACM.