点种法同种异体兔软骨移植修复关节软骨缺损的研究

将 30只新西兰纯种兔共 60个膝关节随机分为 3组 :软骨块点种法移植组、软骨细胞点种法移植组和对照组。分别于术后 2、4、1 2、2 4周取标本行大体、光镜、电镜的组织学动态观察 ,并同时对修复组织进行组织学和组织化学质量评估 ,观察点种法软骨移植术修复透明软骨的效果。结果显示 :2种点种法移植组均能获得透明软骨修复 ,而对照组缺损区仅为纤维组织填充 ,并且点种法移植组兔各期平均组织学和组织化学得分均高于对照组 (P <0 .0 1 )。提示 :点种法软骨移植能获得透明软骨修复 ,尤其适用于大面积软骨缺损。

Study on Point Implantation with Allograft Rabbit Cartilage for Repairing Articular Cartilage Defects

A novel method of cartilage transplantation, point implantation, have been developed for repairing articular cartilage defects. 30 New Zealand rabbits were divided into three groups randomly: In group Ⅰ, the new born allograft cartilage masses were transplanted into the holes in the defect which was made previously. In group Ⅱ, The cultured new born allograft chondrocytes were transplanted like group Ⅰ, and then the holes were covered by collagen gel. In group Ⅲ, The holes in the defect were left empty. Healing of the defects was assessed by gross examination、 light microscopy、 histological histochemical scoring and electron microscopy at 2、4、12 and 24 weeks. Twenty four weeks after the transplantation, the defects were filled with hyaline cartilage, while the defects without transplantation healed with fiber tissue and fibrocartilage. The histologic quality scores of the repair tissue were significantly better in all knees in which defects were treated with point implantation compared to knees in which defects were treated with drilling only. Point Implantation can obtain satisfactory hyaline cartilage repair when used in some large area defects.