| 成年大鼠心肌细胞培养方法的建立和形态学观察 |
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| 引用本文： | 许秀芳,李温斌,陈宝田,吕燕宁,赵莉敏,陈燕,Xu Xiufang,Lü Yanning,Zhao Limin,Chen Yan,Li Wenbin,Chen Baotian.成年大鼠心肌细胞培养方法的建立和形态学观察[J].首都医学院学报,2000,21(2):104-107. |
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| 作者姓名： | 许秀芳 李温斌 陈宝田 吕燕宁 赵莉敏 陈燕 Xu Xiufang Lü Yanning Zhao Limin Chen Yan Li Wenbin Chen Baotian |
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| 作者单位： | 北京心肺血管疾病研究所细胞免疫室(许秀芳，吕燕宁，赵莉敏，陈燕)，首都医科大学附属北京安贞医院心外科(李温斌，陈宝田) |
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| 摘 要： | 为探索稳定的成年大鼠心肌细胞的分离和培养方法 ,应用生物酶 ( 5g/L胰蛋白酶及 1 g/L胶原酶 )灌注法分离成年大鼠心肌细胞 ,用形态学及台盼蓝染色法鉴定分离的心肌细胞 ,并在光镜和电镜下观察和摄像记录。结果 :心肌细胞的存活率为 91 .7% ;存活的心肌细胞静止地贴在培养板底上 ,细胞完整 ,呈杆状 ,长宽比约为 4～ 6∶1。结果提示 :该方法是比较理想的心肌细胞培养法。
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| 关 键 词： | 成年大鼠心肌细胞 胰蛋白酶 胶原酶 分离技术 |
| 收稿时间： | 1999-03-09 |
Morphology Study and Isolated and Cultured Method of Adult Myocytes |
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| Xu Xiufang,Zhao Limin,Chen Yan,Li Wenbin,Chen Baotian.Morphology Study and Isolated and Cultured Method of Adult Myocytes[J].Journal of Capital University of Medical Sciences,2000,21(2):104-107. |
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| Authors: | Xu Xiufang Zhao Limin Chen Yan Li Wenbin Chen Baotian |
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| Institution: | 1. Beijing Heart Lung and Blood Vessel Institute;2. Department of Heart Surgery of Beijing Anzhen Hospital, Affiliate of Capital University of Medical Sciences |
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| Abstract: | In order to build method of a stable isolation and culture of adult myocytes, adult rat myocytes were isolated with bioenzymes (including 5 g/L trypsin and 1 g/L colleagase) perfusing tissue through acceding aorta of the heart. Calcium-tolerant myocytes were harvested, purifyed and cultured with DMEM culture medium . The myocytes quality was evaluated with morphology and trypan blue dye. States of myocytes growing in culture were observed with optical microscope and electron microscope photography. Result: Survival rate of aduld rat myocytes was 91.7%, survival myocytes were motionlessly attached to the bottom of culture plates, they were intact and rod-shaped, their length/width ratio was 4～6: 1. Conclusion: The method of isolation and culture of adult rat myocytes was successful. |
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| Keywords: | adult rat myocytes trypsin colleagase separation technique |
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