阿尔茨海默病患者血清与脑脊液中多种细胞因子的研究

目的　探讨阿尔茨海默病(Alzheimer’sdisease ,AD)病因及发病机制。方法　采用双抗体夹心酶联免疫吸附试验分别对5 7例患者分为AD组和VaD组进行血清和脑脊液(CSF)肿瘤坏死因子-α(TNF- α)、白细胞介素- 1β(IL- 1β)及白细胞介素- 8(I-L 8)水平检测。对照组2 8例均无神经系统、免疫系统等疾病。结果　(1)AD组CSF中TNF- α水平明显高于VaD组和对照组,差异有显著性意义(P <0 .0 5 ,P <0 .0 1) ;(2 )AD组和VaD组CSF中IL- 1β水平明显高于对照组(P <0 .0 5 ) ,AD组血清中IL -1β水平明显高于VaD组和对照组,差异有显著性意义(P <0 .0 1) ;(3)AD组和VaD组血清和CSF中IL 8均明显高于对照组,差异显著(P <0 .0 5 ,P <0 .0 1) ;(4)AD组患者CSF中的TNF -α、IL -1β及IL -8水平之间呈正相关(r =0 .0 6 ,P <0 .0 5 ) ;(5 )血清或CSF中的TNF- α、IL- 1β及IL -8检测水平与简易智力状态检查表(MMSE)得分之间均无相关性。结论　细胞因子可能参与了AD的病理生理过程。

Research on the levels of TNF-α, IL-1β and IL-8 in serum and CSF of patients with Alzheimer' s disease

Objective To investigate the etiology and pathogenesis of Alzheimer's disease (AD). Methods The levels of TNF-α,IL-1β and IL-8 in serum and cerebrospinal fluid (CSF)of patients with AD were examined respectively by double antibody enzyme linked immunosorbent assay (ELISA).Results (1)The TNF-α level in CSF in AD group was significantly higher than that in VD(vascular dementia) and healthy control groups, with significant difference (P<0.05 or P<0.01);(2)The level of IL-1β in CSF in AD and VD groups was significantly higher than that in the healthy control group,with significant difference (P<0.05). The level of IL-1β in serum in AD was significantly higher than that in VD and healthy control groups, with significant difference (P<0.01).(3) The levels of IL-8 in serum and CSF in AD and VD groups were significantly higher than those in the healthy control group,with significant difference (P<0.05 or P<0.01); (4) A positive correlation existed between the levels of TNF-α,IL-1β and IL-8 in CSF of AD group (r=0.06,P<0.05);(5) The levels of TNF-α,IL-1β and IL-8 in serum or CSF were not correlated with MMSE scores in AD group.Conclusion Measurement of cytokines(CK) may provide theoretical grounds for further shedding light on the CK function in AD pathogenesis,and helps to the diagnosis of AD and early protection from AD.