Histochemical and immunohistochemical study of the glomerular development in human fetuses |
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Authors: | Mara Lúcia Fonseca Ferraz Aline Mara dos Santos Camila Lourencini Cavellani Renata Calciolari Rossi Rosana Rosa Miranda Corrêa Marlene Antônia dos Reis Vicente de Paula Antunes Teixeira Eumenia Costa da Cunha Castro |
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Institution: | (1) Biological Sciences Department, General Pathology Discipline, Triangulo Mineiro Federal University, Rua Frei Paulino 30, Bairro Abadia. Cep 38025-180, Uberaba, Minas Gerais, Brazil |
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Abstract: | Few studies exist that establish the normal morphological patterns of glomerular development, though this is one of the organs
that continues to evolve morphologically during the postnatal period up to 4 weeks after birth. In our study one kidney from
each autopsy of a total of 86 autopsies was analyzed 15 weeks to 40 weeks of gestational age (GA)]. We examined the variation
in the nephrogenic zone thickness, the area and diameter of the glomerular tuft, the area and diameter of the glomerular capsule,
and the immunohistochemical markers, anti-CD31 and anti-CD34 antibodies, which accompany the development of the glomerular
microvasculature. Periodic acid–methenamine silver (PAMS) stain was used for the morphological and morphometrical analyses,
and it was particularly useful in fetuses in which autolysis had occurred. The length of the nephrogenic zone (NZ) decreased
with the increase of the GA (P < 0.001) according to the formula: . The areas of the Bowman capsule (P < 0.0001), the capillary tuft (P < 0.0001), and the capillary tuft diameter (P = 0.00393) of the intermediary glomeruli increased with the advance of GA, with a positive significant correlation. The same
parameters of the juxtamedullary and superficial glomeruli had no correlation with the advance of GA. The cells of the primary
structures in the “S” shape of the primitive nephrons were negative for CD31 and CD34. Staining for both antibodies was found,
for all GAs, in the endothelial cells of the mature glomeruli tuft and in the renal interstitial vessels. The data obtained
in this work contribute to the evaluation of renal maturity in autopsied fetuses and are particularly important in fetuses
when autolysis has occurred, to which the parameters used in this study can also be applied. The establishment of normal morphometric
and immunohistochemical parameters for the evaluation of renal maturity increases the diagnostic precision of renal pathological
alterations in aborted material and perinatal autopsy. |
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Keywords: | Kidney Glomerulogenesis Gestational age Perinatal autopsy |
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