甲状腺相关眼病患者血清中抵抗素水平的测定

目的检测甲状腺相关眼病（TAO）患者血清中抵抗素的表达水平。方法 TAO患者血清样本来自有典型临床症状且近3个月内未服用糖皮质激素和免疫抑制剂的TAO患者35例,按CAS临床活动度评分标准将其分为2组：非活动期（CAS〈4）18例和活动期（CAS≥4）17例,另外选取正常对照组15例。使用ELISA法检测血清中抵抗素蛋白的表达情况。结果活动期与非活动期TAO患者血清中抵抗素水平分别为（12.103±5.042）mg/L和（13.470±3.749）mg/L,差异无统计学意义（t=0.906,P=0.371）;正常对照组血清抵抗素水平为（21.894±10.589）mg/L,非活动期、活动期TAO组血清抵抗素水平分别为（12.103±5.042）mg/L和（13.470±3.749）mg/L,均较正常对照组明显下降（t=3.074,P=0.004;t=3.485,P=0.001）。男女患者血清中抵抗素水平分别为（13.175±4.483）mg/L和（12.335±4.511）mg/L,差异无统计学意义（t=0.552,P=0.585）。随着年龄的增加,血清中抵抗素水平逐渐升高,各年龄组间比较差异有统计学意义（F=24.978,P=0.000）。结论 TAO患者血清中抵抗素水平低于正常人,说明影响血清中抵抗素水平的仍是甲状腺激素,并且血清中抵抗素水平随年龄的增加而升高。

Expression of resistin in serum of TAO patients

BackgroundResistin is the member of human cytokines,but its function is below understood.Research revealed that the expression of resistin in serum is regulated by sexual gland,thyroid hormone.ObjectiveThe goal of this study was to investigate the expression of resistin in the serum of TAO patients.MethodsThe serum samples were obtained from 35 patients with thyroid-associated ophthalmopathy（TAO）.These patients rejected the administration of glucocorticoid and immunity inhibitor within 3 months and were assigned to inactive TAO group（CAS4,18 cases）and active TAO group（CAS≥4,17 cases）.Fifteen normal subjects were as control group.The level of resistin in serum was detected using ELISA kit.Written informed consent was obtained from each individual at initial of the medical process.Results No significant difference was found in serum resistin level between inactive TAO group and active TAO group（12.103±5.042 versus 13.470±3.749）mg/L（t=0.906,P=0.371）.However,serum resistin level was significant declined in inactive TAO group or active TAO group in comparison with normal group（12.103±5.042 versus 21.894±10.589;13.470±3.749 versus 21.894±10.589）mg/L（t=3.074,P=0.004;t=3.485,P=0.001）.No obvious difference was seen between male patients and female patients（13.175±4.483 versus 12.335±4.511）mg/L（t=0.552,P=0.585）.Serum resistin level was gradually elevated with the increase of age in TAO patients（F=24.978,P=0.000）.ConclusionThe serum resistin level of TAO patients is lower than the control subjects,suggesting that the level of serum resistin is regulated by thyroid hormones.The serum resistin level increases with age in TAO patient.