血管内皮细胞生长因子在激素治疗兔关节炎模型过程中的基因表达

目的 通过观察激素治疗兔关节炎模型过程中滑膜组织内血管内皮细胞生长因子（VEGF）的基因表达,进一步探讨激素治疗关节炎的作用机制。方法 采用逆转录－聚合酶链反应（RT－PCR）方法检测激素治疗兔关节炎模型不同阶段[用脂多糖（LPS）后1h,用药后24h、3、7、14、21、28d]滑膜组织中VEGF基因的表达及变化。结果 在激素治疗关节炎的不同时期,VEGF基因表达的程度不同,激素对VEGF有明显的抑制作用。结论 激素通过对VEGF的抑制作用,减轻了关节炎的炎性反应。

Expression of vascular endothelial growth factor in treatment process of rabbit arthritis model with corti-costeroids

Objective To observe the expression of vascular endothelial growth factor (VEGF) in synovial tissue during treatment of rabbit arthritis model with corticosteroids and explore its mechanism.Methods The expression and alteration of VEGF in different periods of treatment process of rabbit arthritis model with corticosteroids (after 1 h ,24 h,3,7,14,21 and 28 d treatment with lipopolysaccharide),were investigated by reverse transcript polymerase chain reaction (RT PCR).Results There were different expressive degrees of VEGF in synovial tissue during each period of the process of rabbit arthritis model by corticosteroid treatment and the expressive degree of VEGF had been strongly inhibited by corticosteroids.Conclusion It is indicated that corticosteroids decrease inflammatory reaction of arthritis by inhibiting the expression of VEGF.