几种抗氧化剂对细胞间粘附分子-1表达的影响

目的 探讨抗氧化剂调节内皮细胞表面的粘附分子ICAM-1表达的分子生物学机制。方法 内皮细胞表面的粘附分子表达用细胞ELISA方法测定,内皮细胞NF-kB的活性用电泳迁移率分析测定。结果 PDTC,DCI和chrysin能明显抑制ICAM-1的表达。Probucol仅在低浓度时对ICAM-1有轻微抑制作用。电泳迁移率分析结果,只有PDTC对NF-kB的活性有抑制作用,其它三种抗氧化药无效。结论 这几种抗氧化药抑制内皮细胞表面粘附分子的表达与它们对核转录因子NF-kB的作用并无必然的联系。

Effects of antioxidants on ICAM-1 expression in human endothelial cells

ve To investigate whether some antioxidants modulate expression of endothelial cell adhesion molecule ICAM - 1 and whether antioxidants modulate expression of endothelial cell adhesion molecules through regulating NF - kB activation. Methods The effect of the antioxidative substances, pyrrolidin dithiocar-bamat ( PDTC), chrysin, dichloroisocoumarin ( DCI), and probucol, on the expression of ICAM - 1 was measured with cell - ELISA. The activation of NF - kB in en-dothelical cell was investigated in a gel shift assay. Results PDTC, DCI and chrysin showed a concentration -dependent inhibition on expression of ICAM - 1, but probucol inhibited ICAM - 1 expression in low dose. Only PDTC inhibited the TNFa - induced activation of NF - kB in endothelial cell. Conclusions The down - regulation of the endothelial cell adhesion molecule expression by antioxidative substances was independent on their effect on NF - kB activation.