载脂蛋白B基因多态性与中青年人颈动脉粥样硬化关系的研究

目的旨在观察载脂蛋白B(apolipoprotein B,apoB)基因多态性与中青年颈动脉粥样硬化的关系。方法用聚合酶链反应(polymerase chain reaction PCR)对84例颈动脉粥样硬化患者及75例健康对照组的apoB基因EcoRI酶切位点限制性片段长度多态性(restriction fragment length polymorphism RELP)进行研究。利用二维彩色多普勒超声,检测两组颈总动脉内膜—中膜厚度(intima-media thickness,IMT)及斑块指数(plaque index,PI)。结果颈动脉粥样硬化组及健康对照组均以E E 基因型为主,E E-杂合子少见,E-E-基因型最少;颈动脉粥样硬化组E-等位基因频率高于健康对照组,分别为0.107和0.06(P>0.05)。颈动脉粥样硬化组含E-基因型者,血清TC、LDL-c、甘油三酯(triglycerid TG)有增加趋势,但各指标与E E 基因型间差异不显著(P>0.05)。同样,含E-基因型者IMT、CA-PI较健康对照组有增加趋势,但差异不显著(P>0.05)。结论推测EcoRI酶切位点多态性与颈动脉粥样硬化无明显关联。

The relation between polymorphisms of apolipoprotein B gene and caroltid atherosclerosis in the youth

Objective This syudy aimed to explore association of variance at EcoRI site of apoB gene and cartiod atherosclerosis in Chinese youth population.Methods Using polymerase chain reaction(PCR) techniques,we studied the restriction fragment length polymorphisms(RFLP) at EcoRI site of apoB gene in 84 patients with cartiod atherosclerosis and 75 healthy age,sex-matched individuals from a population in Ningxia.And measured cartiod intima-media thickness(IMT) and plaque index(PI) in the cartiod by ultrasonography.Results In both cartiod atherosclerosis group and control group,E~ E~ genotypes were the most frequent one.There was no higher frequence of rare allele E~-was found in the cartiod atherosclerosis group as compared with the control group(0.107vs 0.06,P>0.05);in the E~ E~-and E~-E~ genotypes in the cartiod atherosclerosis group,the TC and LDL-c have a trend of increase,but the difference was not significantly.The same result was found in the statistics of IMT and CA-PI.Conclusion The polymorphisms of EcoRI may be not associated with cartiod atherosclerosis.