| 用单克隆抗体酶联免疫法检测临床标本中单纯疱疹病毒抗原 |
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| 引用本文： | 李连青,朱庆义,赵瑞珍,贺润花,秦克锋,喻启桂,汪美先.用单克隆抗体酶联免疫法检测临床标本中单纯疱疹病毒抗原[J].细胞与分子免疫学杂志,1992(2). |
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| 作者姓名： | 李连青 朱庆义 赵瑞珍 贺润花 秦克锋 喻启桂 汪美先 |
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| 作者单位： | 山西省妇幼保健院，山西省妇幼保健院，山西省妇幼保健院，山西省妇幼保健院，第四军医大学微生物学教研室，第四军医大学微生物学教研室，第四军医大学微生物学教研室 太原 030013，太原 030013，太原 030013，太原 030013，西安 710032，西安 710032，西安 710032 |
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| 摘 要： | 本文报告在太原市6所医院从各类感染性疾病中采取各种临床标本2067份,正常妇女阴道分泌物72份,用ELISA法快速分型检测单纯疱疹病毒抗原(HSV-Ag),总阳性率为24.5％,其中HSV-1占4.4％,HSV-2占20.1％;正常对照总阳性率为7.0％,HSV-1占1.4％,HSV-2占5.6％。HSV-Ag阳性率和型分布因感染部位而不同:肺部感染以HSV-1居多,生殖器疱疹、疱疹性脑炎、宫颈癌和胎儿畸型均以HSV-2为主。
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| 关 键 词： | 单纯疱疹病毒 抗原 抗体 单克隆 酶联免疫吸附试验 |
Detection of HSV antigen in clinical specimens by McAb-ELISA |
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| Li Lianqing,Zhu Qingyi,Zhao Ruizhen,He Runhua,Qin Kefeng,Yu Qigui and Wang Meixian.Detection of HSV antigen in clinical specimens by McAb-ELISA[J].Journal of Cellular and Molecular Immunology,1992(2). |
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| Authors: | Li Lianqing Zhu Qingyi Zhao Ruizhen He Runhua Qin Kefeng Yu Qigui and Wang Meixian |
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| Abstract: | In the present study, a total of 2067 clinical specimens and 72 normal female Taginal secretions were collected from 6 hospitals in Taiyuan for detection of type-specific herpes simplex virus antigen ( HSV-Ag ) by enzyme-linked immunosorbe-nt assay using; monoclonal antibodies ( McAb-ELISA ). The findings showed that the total positive rate was 24.5% ( HSV-14.4%; HSV-220.1% ) while that of the control was 7.0%. The results from HSV-type identification demonstrated that genital infections ( cervicitis, vaginitis, and vulvitis ) , cervical cancer, fetal anomaly, and viral encephalitis were predominated by HSV-2 while pulmonary infection was mainly HSV-1. The McAb-ELISA was shown to be a simple, rapid and sensitive method and might be used an effective alternative to routine cell culture for diagnosis of HSV infection. |
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| Keywords: | herpes simplex virus intection antibody monoclonal enzyme-linked immunosorbent assay |
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