| 自制电致细胞融合仪的初步应用 |
| |
| 引用本文： | 余海,胡汛,郑树,华蕴博,郭一舒,刘克斌. 自制电致细胞融合仪的初步应用[J]. 浙江大学学报(医学版), 1989, 0(4) |
| |
| 作者姓名： | 余海 胡汛 郑树 华蕴博 郭一舒 刘克斌 |
| |
| 作者单位： | 浙江医科大学肿瘤研究所，浙江医科大学肿瘤研究所，浙江医科大学肿瘤研究所，浙江医科大学生物医学工程系，浙江医科大学生物医学工程系，浙江农大园艺系 |
| |
| 摘 要： | 应用自制电致细胞融合仪,在显微镜直视下,可观察到小鼠胸腺细咆时伊红染料的电渗入效应、在电极间各类细胞的电介质电泳现象以及植物原生质体间的电融合过程.小鼠脾细胞和骨髓瘤FO细胞电融合后,转移至HAT培养基继续培养后,有细胞克隆形成,此系电融合致杂交瘤形成的可靠依据.以上结果表明,自制电融合仪能满足细胞工程研完的基本要求.
|
| 关 键 词： | 细胞融合/仪器和设备 电泳 电渗入 |
PRELIMINARY APPLICATION OF SELF-MADE ELECTROFUSION APPARATUS XR-1 |
| |
| Yu Hai,Hu Xun,Zheng Shu,Cancer InstituteHua Yunbo,Gu Yishu,Dept of Biomedical EngineeringLiu Kebin,Dept of Horticulture,Zhejiang Agricultural University. PRELIMINARY APPLICATION OF SELF-MADE ELECTROFUSION APPARATUS XR-1[J]. Journal of Zhejiang University. Medical sciences, 1989, 0(4) |
| |
| Authors: | Yu Hai Hu Xun Zheng Shu Cancer InstituteHua Yunbo Gu Yishu Dept of Biomedical EngineeringLiu Kebin Dept of Horticulture Zhejiang Agricultural University |
| |
| Abstract: | A series of cytobiologieal experiments was carried out with a self-made electrofusion apparatus XR-1. The electropermeabilization of eosine dye by mouse thymocytes, the dielectrophoresis of various types of cells, electrofu-sion between protoplasts of the domesticated tomato (Lycopersicon esculen-tum)and the wild tomato (L.hirsutum) were directly monifored under the inverse microscope. Seven days after the fusion of mouse spleen cells and mouse myeloma FO cells, the hybridoma cell clones were formed in HAT mediam which was a strong evidence of the success in electrofusion bet-ween these two types of cells. The above rasults indicate that the self-made electrofusion apparatus is able to meet the basic requirements of electro-fusion and electropermeabilization. |
| |
| Keywords: | Cellfusion/instruml Electrophoresis Electropermeabilization |
| 本文献已被 CNKI 等数据库收录！ |
|
