Effect of isoproterenol on the uptake of [14C]glucose into glial cells

The effect of isoproterenol on the uptake of [14C]glucose into cultured glial cells was investigated in the present study. Isoproterenol markedly stimulated the uptake of [14C]glucose 30 min after incubation. This action was produced in a dose-dependent manner. A positive correlation between the increase of [14C]glucose uptake and the stimulation of adenylate cyclase induced by isoproterenol was obtained (r = 0.99). The effect of isoproterenol was reduced by the beta-adrenergic blockers but not by other blockers, indicating the selective action of isoproterenol. The results obtained suggest that isoproterenol stimulates the uptake of glucose into glial cells through the activation of beta-adrenoceptors which are linked to adenylate cyclase.