| 改良的兔晶体上皮细胞体外培养 |
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| 引用本文： | 石广森,刘平.改良的兔晶体上皮细胞体外培养[J].中国伤残医学,2006,14(2):23-25. |
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| 作者姓名： | 石广森 刘平 |
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| 作者单位： | 哈尔滨医科大学附属第一医院眼科医院,黑龙江,哈尔滨,150001 |
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| 摘 要： | 目的:建立一种改良的兔晶状体上皮细胞体外培养的方法,提高晶体上皮细胞原代培养的成功率。方法:利用改良消化法对兔晶体上皮细胞进原代培养,对培养的细胞进行形态学观察。结果:接种48小时~72小时后即可见上皮细胞贴壁生长,具备上皮细胞的形态特点;约7天后融合。传至5代后,细胞明显成纤维细胞化。结论:此种方法经济简便,可为各种实验提供兔晶体上皮细胞。
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| 关 键 词： | 晶体上皮细胞 细胞培养 晶状体 |
| 文章编号： | 1673-6567（2006）02-0023-03 |
| 收稿时间： | 2005-12-24 |
| 修稿时间： | 2005-12-24 |
A Developed Procedure To Culture Rabbit Lens Epithelial Cells |
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| SHI Guang-sen,LIU Ping.A Developed Procedure To Culture Rabbit Lens Epithelial Cells[J].Chinese JOurnal of Trauma and Disability Medicine,2006,14(2):23-25. |
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| Authors: | SHI Guang-sen LIU Ping |
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| Institution: | Department of Ophthamology, the First Hospital of Harbin Meidical University, Harbin, Heilongjiang Province, 150001 China |
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| Abstract: | Objective: To establish a developed procedure to culture rabbit lens epithelial cells for increasing the success rate of its primary culture. Methods: Using the developed procedure to culture rabbit lens epithelial cells, and the cultured cells were examined morphologically. Result:Primary culture for 48 to 72 hours, the lens epithelial grew, they maintained typical epithelial morphology and reach confluence after about 7 days. After passage 5 , the morphology of cells was transformed to fibroblast apparently. Conclusion: This technique is simple and lower cost to provide rabbit lens epithelial cells for use in various experiments . |
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| Keywords: | lens epithelial cell cell culture lens crystalline |
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