Coding single nucleotide polymorphism in the high-affinity immunoglobulin E receptor b chain (Fc"RI-b) gene is associated with immunoglobulin E receptor-mediated histamine release from basophils |
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| Authors: | Y.-K. Kim&dagger &Dagger ,S.-Y. Oh&dagger &Dagger ,H.-B. Oh§ ,S.-Y. Chun&dagger &Dagger ,S.-H. Cho&dagger &Dagger ,Y.-Y. Koh¶ ,K.-U. Min&dagger &Dagger , Y.-Y. Kim&dagger &Dagger |
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| Affiliation: | Department of Internal Medicine, Seoul National University College of Medicine, Korea. youyoung@plaza.snu.ac.kr |
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| Abstract: | BACKGROUND: Our previous work on linkage analysis showed that histamine release from basophils to anti-IgE stimuli was linked to the gene marker of chromosome 11q13, where the beta chain of the high-affinity receptor for IgE (FcepsilonRI-beta) is located. OBJECTIVE: To evaluate the association between FcepsilonRI-mediated histamine release from basophils and four bi-allelic single nucleotide polymorphisms of the FcepsilonRI-beta gene. METHODS: Phenotypes of asthma, such as maximal histamine release from basophils and atopy, were measured from 80 randomly recruited asthmatic children. Polymorphisms of the FcepsilonRI-beta gene were determined by PCR-based methods. RESULTS: The polymorphism in exon 7, resulting in Glu to Gly substitution, was significantly associated with histamine release from basophils to anti-IgE stimuli, but not with total IgE levels and skin test responses to aeroallergens. CONCLUSION: This study supports a role for the FcepsilonRI-beta gene in the expression of high affinity IgE receptor-mediated histamine release from basophils. |
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| Keywords: | asthma atopy basophil histamine release FcεRI-β gene polymorphism |
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