Affinity hydrogels for controlled protein release using nucleic acid aptamers and complementary oligonucleotides |
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Authors: | Soontornworajit Boonchoy Zhou Jing Snipes Matthew P Battig Mark R Wang Yong |
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Affiliation: | Department of Chemical, Materials & Biomolecular Engineering, University of Connecticut, Storrs, CT 06269-3222, USA. |
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Abstract: | Biomaterials for the precise control of protein release are important to the development of new strategies for treating human diseases. This study aimed to fundamentally understand aptamer--protein dissociation triggered by complementary oligonucleotides, and to apply this understanding to develop affinity hydrogels for controlled protein release. The results showed that the oligonucleotide tails of the aptamers played a critical role in inducing intermolecular hybridization and triggering aptamer--protein dissociation. In addition, the attachment of the oligonucleotide tails to the aptamers and the increase of hybridizing length could produce a synergistic effect on the dissociation of bound proteins from their aptamers. More importantly, pegylated complementary oligonucleotides could successfully trigger protein release from the aptamer-functionalized hydrogels at multiple time points. Based on these results, it is believed that aptamer-functionalized hydrogels and complementary oligonucleotides hold great potential of controlling the release of protein drugs to treat human diseases. |
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