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New cyclomyrsinol diterpenes from Euphorbia aellenii with their immunomodulatory effects |
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| Authors: | Mustafa Ghanadian M. Iqbal Choudhary Abdul Majid Ayatollahi M. Ahmad Mesaik Omer Mohamed Abdalla Suleiman Afsharypour |
| Affiliation: | 1. Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences , Isfahan , Islamic Republic of Iran ghannadian@gmail.com;3. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi , Karachi , 75270 , Pakistan;4. Phytochemistry Research Center and School of Pharmacy, Shahid Beheshti University of Medical Sciences , Tehran , Islamic Republic of Iran;5. Department of Pharmacognosy , Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences , Isfahan , Islamic Republic of Iran |
| Abstract: | Chloroform–acetone extract of the aerial parts of Euphorbia aellenii Rech. f. (Euphorbiaceae) was investigated for its diterpenoidal constituents. This led to the isolation of two new and one known cyclomyrsinol-type diterpenes 1–3. The structures were elucidated on the basis of 1D and 2D 1H and 13C NMR techniques, and in vitro immunomodulatory activity was evaluated by standard proliferation of human peripheral blood lymphocytes. Results showed that all the three compounds were found to inhibit lymphocyte proliferation significantly (p < 0.05) at 50 μg/ml concentration. Among them, compound 2 showed more activity against phytohemagglutinin-activated T-cell proliferation with an IC50 of 40.4 ± 9.35 μg/ml. |
| Keywords: | Euphorbiaceae Euphorbia aellenii cyclomyrsinol immunomodulation |