Microbial transformation of diosgenin by filamentous fungus Cunninghamella echinulata |
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| Authors: | Xiao Xiao Xi-Kui Liu Shao-Bin Fu |
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| Affiliation: | 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College , Beijing, 100193, China;2. Kunming Institute of Botany, Chinese Academy of Sciences , Kunming, 650204, China;3. Kunming Institute of Botany, Chinese Academy of Sciences , Kunming, 650204, China;4. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College , Beijing, 100193, China |
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| Abstract: | Microbial transformation of diosgenin (1) by suspended-cell cultures of the filamentous fungus Cunninghamella echinulata CGMCC 3.2000 was investigated. Incubation of the substrate diosgenin (1) with this fungus led to the isolation of three products: two known compounds, (25R)-spirost-5-en-3β,7β,12β-triol (2) and (25R)-spirost-5-en-3β,7β,11α-triol (3), and a new compound (25R)-spirost-5-en-3β,7α,11α-triol (4). The structural elucidations of the three compounds were achieved mainly by the MS, 1D and 2D NMR spectroscopic methods and comparison with known compounds. C. echinulata CGMCC 3.2000 has not been used before in the biotransformation of diosgenin (1). |
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| Keywords: | microbial transformation biotransformation hydroxylation diosgenin Cunninghamella echinulata CGMCC 3.2000 |
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