A novel indole alkaloid from deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth |
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| Authors: | Lin Chen Xi-Xiang Tang Min Zheng Zhi-Wei Yi Xiang Xiao |
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| Institution: | 1. Medical College, Xiamen University , Xiamen, 361005, China;2. School of Life Science, Xiamen University , Xiamen, 361005, China;3. Key Laboratory of Marine Biogenetic Resources , Third Institute of Oceanography, State Oceanic Administration , Xiamen, 361005, China;4. Key Laboratory of Marine Biogenetic Resources , Third Institute of Oceanography, State Oceanic Administration , Xiamen, 361005, China |
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| Abstract: | To explore secondary metabolites in deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth, different kinds of chromatography methods were used in the isolation procedures, while the structures of the isolated compounds were assigned based on the MS analysis and their 1H and 13C NMR spectra including 2D NMR techniques such as COSY, HMQC, and HMBC experiments. As a result, a novel compound was isolated and characterized as N-{1-4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2,2-dichloroacetamide (1). In addition, eight known compounds were also obtained. Fatty acid amide hydrolase and monoacylglycerol lipase were used to screen analgesic activity, and the new compound showed analgesic activity to some extent in pharmacological test. |
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| Keywords: | deep-sea sediment metagenomic clone N-{1-[4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2 2-dichloroacetamide indole alkaloids |
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