Triterpenoid saponins from the roots of Clematis chinensis Osbeck |
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Authors: | Li-Fang Liu Xiao-Li Ma Yu-Xin Wang Feng-Wen Li Yun-Man Li Zhu-Qing Wan |
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Affiliation: | 1. The Key Laboratory of Modern Chinese Medicines (Ministry of Education) and Department of Pharmacognosy , China Pharmaceutical University , Nanjing, 210038, China liulifang69@hotmail.com;3. The Key Laboratory of Modern Chinese Medicines (Ministry of Education) and Department of Pharmacognosy , China Pharmaceutical University , Nanjing, 210038, China;4. School of Traditional Chinese Pharmacy, China Pharmaceutical University , Nanjing, 210038, China;5. Department of Pharmacology , China Pharmaceutical University , Nanjing, 210038, China |
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Abstract: | A new triterpenoid saponin named clematichinenoside AR2, along with the six known compounds, was isolated and characterized from Clematis chinensis Osbeck (Ranunculaceae), a commonly used traditional Chinese medicine with anti-inflammatory and anti-rheumatoid activities. The structure of the new saponin was elucidated as 3-O-β-[(O-α-l-rhamnopyranosyl-(1 → 6)-O-β-d-glucopyranosyl-(1 → 4)-O-β-d-glucopyranosyl-(1 → 4)-O-β-d-ribopyranosyl-(1 → 3)-O-α-l-rhamnopyranosyl-(1 → 2)-α-l-arabinopyranosyl)oxy]olean-12-en-21α-hydroxy-28-oic acid-O-α-l-rhamnopyranosyl-(1 → 4)-O-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl ester (1) by spectral analysis and chemical methods. The effects of two major saponins (clematichinenosides AR and AR2) on the secretion of TNF-α in murine peritoneal macrophages induced by lipopolysaccharides were further investigated. The result indicated that a majority of triterpenoid saponins of this herb may be useful in the exploration of lead compounds for the treatment of some autoimmune diseases. |
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Keywords: | Ranunculaceae Clematis chinensis Osbeck triterpenoid saponins clematichinenoside AR clematichinenoside AR2 TNF-α |
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