Atypical apoptosis in L929 cells induced by evodiamine isolated from Evodia rutaecarpa |
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| Authors: | Ying Zhang Qi-Hui Zhang Li-Jun Wu Shin-Ichi Tashiro Satoshi Onodera Takashi Ikejima |
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| Institution: | 1. China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University , Shenyang, 110016, China ikejimat@vip.sina.com;3. Department of Phytochemistry , Shenyang Pharmaceutical University , 110016, Shenyang, China;4. Department of Clinical and Biomedical Sciences , Showa Pharmaceutical University , 194-8543, Tokyo, Japan;5. China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University , Shenyang, 110016, China |
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| Abstract: | Two alkaloids, evodiamine and rutaecarpine, isolated from the dried fruits of Evodia rutaecarpa Bentham were evaluated in vitro for antiproliferation activity on tumor cells versus human peripheral blood mononuclear cells (PBMC). Evodiamine had more potent cytotoxic effects on five tumor cell lines (human malignant melanoma A375-S2, human cervical cancer HeLa, human breast adenocarcinoma MCF7, human acute monocytic leukemia THP-1, murine fibrosarcoma L929) than rutaecarpine. Moreover, evodiamine did not affect PBMC viability for a 36?h culture period. Although apoptotic bodies were observed in evodiamine-treated L929 cells stained with Hoechst 33258, DNA fragmentation as a hallmark of apoptosis was not found. Caspases were involved in the protection of L929 cells against cell death. Evodiamine initiated atypical apoptosis in L929 cells by cycle arrest at the G0/G1 phase. |
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| Keywords: | Evodiamine Rutaecarpine Apoptosis Caspases |
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