Inhibitory effect of 1,25-dihydroxyvitamin D3 on Porphyromonas gingivalis-induced inflammation and bone resorption in vivo |
| |
| Affiliation: | 1. School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, USA;2. Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA;3. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA;4. Department of Periodontics, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78284, USA;1. Department of Periodontology, Tokyo Dental College, Tokyo 101-0061, Japan;2. Department of Materials Science and Technology, Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan;3. Department of Microbiology, Tokyo Dental College, Tokyo 101-0061, Japan;4. Oral Health Science Center, Tokyo Dental College, Tokyo 101-0061, Japan;5. Graduate School of Technology, Niigata Institute of Technology, Niigata 945-1195, Japan;6. Laboratory of Chemistry, Tokyo Dental College, Tokyo 101-0062, Japan;1. College of Life Science and Technology, Central South University of Forestry and Technology, 410004 Changsha, China;2. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, 410082 Changsha, China;1. Postgraduate student, Master Degree Program in Dentistry, Potiguar University (Laureate International Universities), Natal, Rio Grande do Norte, Brazil;2. Assistant professor, Department of Oral Medicine and Radiology, School of Dentistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil;3. Assistant professor, Department of Orthodontics, State University of Rio Grande do Norte, Caico, Brazil;4. Assistant professor, Division of Orthodontics, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada;1. Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand;2. Department of Conservative Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand;3. Common Oral Diseases and Epidemiology Research Center and the Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Thailand;4. Department of Oral Microbiology and Immunology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden;1. Departamento de Odontopediatria e Ortodontia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil;2. Escola Politécnica da UFRJ, Laboratório de Instrumentação Nuclear, Rio de Janeiro, RJ, Brazil;3. Departamento de Morfologia, Universidade Federal da Paraíba (UFPB), João Pessoa, Brasil, Brazil |
| |
| Abstract: | ObjectiveTo investigate whether intragastric administration of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) could inhibit the bone resorption and inflammation in a mouse calvarial model infected by Porphyromonas gingivalis (P. gingivalis).DesignLive P. gingivalis ATCC 33277 was injected once daily for 6 days into the subcutaneous tissue overlying the calvaria in mice. At the same time, 1,25(OH)2D3 (50 μg/kg per day) was administered by gavage for 9 days, starting 3 d before the infection. Mice were killed under ether anesthesia 8 h after the last injection of P. gingivalis. Micro-computed tomography scanning was used to evaluate calvarial bone loss. Tartrate-resistant acid phosphatase was used to detect osteoclast activity. Real-time PCR was used to assess the mRNA expressions of OPG, RANKL, c-Fos, NFATc1, CTSK and TRAP in calvarial bone and IL-6, IL-10, IL-1β, IL-12p40 and TNF-α in soft tissue. The levels of serum IL-6, IL-10 were determined by ELISA.Results1,25(OH)2D3 treatment apparently attenuated bone resorption in P. gingivalis-induced mouse calvarial model and markedly reduced the number of osteoclasts. The expression levels of RANKL and osteoclast-related genes such as c-Fos, NFATc1, CTSK and TRAP were also decreased by 1,25(OH)2D3. Besides, 1,25(OH)2D3 inhibited the expression of pro-inflammatory cytokines IL-6, IL-12p40 and TNF-α and enormously elevated the expression of anti-inflammatory cytokine IL-10.Conclusion1,25(OH)2D3 may decrease bone resorption in vivo via suppressing the expression of osteoclast-related genes and its anti-inflammatory properties. |
| |
| Keywords: | Bone resorption Inflammation |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
