Insulin-like growth factor 1 promotes the proliferation and committed differentiation of human dental pulp stem cells through MAPK pathways |
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| Affiliation: | 1. Department of Mechanical Engineering, National Taiwan University, Taipei, Taiwan;2. School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan;3. School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan;4. Department of Dentistry, Taipei Medical University Hospital, Taipei, Taiwan;5. Department of Materials Science and Engineering, Feng Chia University, Taichung, Taiwan;6. Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan |
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| Abstract: | ObjectivesInsulin-like growth factor 1 (IGF-1) is a broad-spectrum growth-promoting factor that plays a key role in natural tooth development. Human dental pulp stem cells (hDPSCs) are multipotent and can influence the reparative regeneration of dental pulp and dentin. This study was designed to evaluate the effects of IGF-1 on the proliferation and differentiation of human dental pulp stem cells.MethodsHDPSCs were isolated and purified from human dental pulps. The proliferation and osteo/odontogenic differentiation of hDPSCs treated with 100 ng/ml exogenous IGF-1 were subsequently investigated.ResultsMTT assays revealed that IGF-1 enhanced the proliferation of hDPSCs. ALP activity in IGF-1-treated group was obviously enhanced compared to the control group from days 3 to 9. Alizarin red staining revealed that the IGF-1-treated cells contained a greater number of mineralization nodules and had higher calcium concentrations. Moreover, western blot and qRT-PCR analyses demonstrated that the expression levels of several osteogenic genes (e.g., RUNX2, OSX, and OCN) and an odontoblast-specific marker (DSPP) were significantly up-regulated in IGF-1-treated hDPSCs as compared with untreated cells (P < 0.01). Interestingly, the expression of phospho-ERK and phospho-p38 were also up-regulated, indicating that the MAPK signaling pathway is activated during the differentiation of hDPSCs.ConclusionsIGF-1 can promote the proliferation and osteo/odontogenic differentiation of hDPSCs by activating MAPK pathways. |
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| Keywords: | Insulin-like growth factor 1 Dental pulp stem cells Osteogenesis Odontogenesis Proliferation Differentiation |
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