Efficacy of doxycycline release collagen membrane on surgically created and contaminated defects in rat tibiae: A histopathological and microbiological study |
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| Institution: | 1. Department of Oral Implantology, Faculty of Dentistry, Yeditepe University,Göztepe, Istanbul, Turkey;2. Department of Oral and Maxillofacial Surgery, Trakya University, Edirne, Turkey;3. Department of Oral and Maxillofacial Surgery, Yeditepe University, Göztepe, Istanbul, Turkey;4. Department of Oral Implantology, Faculty of Dentistry, Yeditepe University, Göztepe, Istanbul, Turkey;5. Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Kay??da?i, Istanbul, Turkey;6. Department of Pathology, Faculty of Veterinary, Istanbul University, Avcilar, Istanbul, Turkey;7. Department of Biological Sciences and Bioengineering, Faculty of Engineering and Natural Sciences, Sabanci University, Tuzla, Istanbul, Turkey;8. Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Kay??da?i, Istanbul, Turkey;9. Department of Pathology, Faculty of Veterinary,Istanbul University, Avcilar, Istanbul, Turkey;1. Department of Radiology, Medstar Georgetown University Hospital, 3800 Reservoir Rd NW, CG201, Washington, DC 20007, USA;2. Department of Neurosurgery, Medstar Georgetown University Hospital, 3800 Reservoir Rd NW, 7PHC, Washington, DC 20007, USA;1. Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan;2. Department of Biochemistry, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan;3. Department of Oral Pathology, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan;4. Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan;5. University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan;6. Department of Biotechnology, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan;1. Department of Biotechnology, The Catholic University of Korea, Bucheon-si 420-743, Republic of Korea;2. Department of Biomedical Engineering and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea;1. Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey;2. Department of Endodontics, Faculty of Dentistry, Bülent Ecevit University, Zonguldak, Turkey;1. Department of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, NY, USA;2. Department of Periodontics, Faculty of Dentistry, Benghazi University, Benghazi, Libya;3. Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA;4. Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Institute of Dentistry, University of Helsinki, Helsinki, Finland;5. Division of Periodontology, Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden;6. Department of Periodontics, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada;7. Department of Periodontology, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel;8. Department of Cariology and Comprehensive Care, College of Dentistry, New York University, New York City, NY, USA;9. Department of General Dentistry, School of Dental Medicine, Stony Brook University, Stony Brook, NY, USA |
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| Abstract: | BackgroundThe effects of systemic antibiotics on controlling infective pathogens after guided bone regeneration(GBR) procedures especially in membrane exposures are limited. However, local administrations of antibiotics are rare in GBR techniques.AimThe aim of this study was to investigate the osteogenesis potential and the antibacterial effect of a doxycycline releasing collagen membrane in surgically created and contaminated defects in rat tibiae.Material and methodsDefects were created in 20 rats that were randomly divided in to two groups: control group (defect contaminated by Porphyromonas gingivalis, filled with bone graft and covered by collagen membrane); test group (defect contaminated by P. gingivalis filled with bone graft and covered by collagen membrane containing 1 mg/cm2 doxycycline. Animals were sacrificed post surgically on the 14th day for microbiologic evaluation and on the 28th day for histopathological evaluation.ResultsThe degree of osteogenesis in the test group was seen to be significantly higher than control group (p: 0.011; p < 0.05). Furthermore in test group, no bacterial growth was observed. The bacteria counts were determined between 1 × 104 and 268 × 104 CFU/g with a median of 1.32 × 104 for control group.ConclusionsWithin the limitations of this study, the results of the present study suggests that the use of a doxycycline releasing membrane has a positive effect on contaminated GBR procedures for limiting P. gingivalis infections leading to bone formation following GBR procedures in a rat model. |
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| Keywords: | Doxycycline Rat tibiae Guided bone regeneration |
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