In vitro dentine remineralization with a potential salivary phosphoprotein homologue |
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| Institution: | 1. Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, ICB/UFMG, Avenida Presidente Antônio Carlos 6627, 31.270-9010 Belo Horizonte, MG, Brazil;2. Department of Pediatric Dentistry and Orthodontics, Faculty of Dentistry, Federal University of Minas Gerais, Avenida Presidente Antônio Carlos 6627, 31.270-9010 Belo Horizonte, MG, Brazil;3. Department of Biological Sciences, School of Dentistry of Bauru, São Paulo University, FOB/USP, Al. Octávio Pinheiro Brisola 9-75, CEP 17012-901 Bauru, SP, Brazil;4. Department of Biological Sciences, School of Dentistry of Bauru, São Paulo University, FOB/USP, Al. Octávio Pinheiro Brisola 9-75, CEP 17012-901 Bauru, SP, Brazil;5. Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, ICB/UFMG, Avenida Presidente Antônio Carlos 6627, 31.270-9010 Belo Horizonte, MG, Brazil;6. Laboratory of Pulmonary Immunology and Mechanics, Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, ICB/UFMG, Avenida Presidente Antônio Carlos 6627, 31.270-9010 Belo Horizonte, MG, Brazil;7. Department of Physics and Chemistry, Faculty of Pharmaceutical Science of Ribeirão Preto, São Paulo University, Avenida do Café, s/n, Cidade Universitária, 14040-903 Ribeirao Preto, SP, Brazil;8. Department of Oral Pathology and Surgery, Faculty of Dentistry, Federal University of Minas Gerais, ICB/UFMG, Avenida Presidente Antônio Carlos 6627, 31.270-9010 Belo Horizonte, MG, Brazil |
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| Abstract: | ObjectiveAdvantages of introducing a salivary phosphoprotein homologue under standardized in vitro conditions to simulate the mineral-stabilizing properties of saliva have been proposed. This study longitudinally investigates the effects of casein, incorporated as a potential salivary phosphoprotein homologue in artificial saliva (AS) solutions with/without fluoride (F) on in vitro dentine lesion remineralization.DesignThin sections of bovine root dentine were demineralized and allocated randomly into 6 groups (n = 18) having equivalent mineral loss (ΔZ) after transverse microradiography (TMR). The specimens were remineralized using AS solutions containing casein 0 μg/ml, F 0 ppm (C0–F0); casein 0 μg/ml, F 1 ppm (C0–F1); casein 10 μg/ml, F 0 ppm (C10–F0); casein 10 μg/ml, F 1 ppm (C10–F1); casein 100 μg/ml, F 0 ppm (C100–F0) or casein 100 μg/ml, F 1 ppm (C100–F1) for 28 days with TMR taken every 7 days.ResultsSurface mineral precipitation, evident in group C0–F1, was apparently inhibited in groups with casein incorporation. Repeated measures ANOVA with Bonferroni correction revealed higher ΔZ for non-F and non-casein groups than for their counterparts (p < 0.001). Subsequent multiple comparisons showed that mineral gain was higher (p < 0.001) with 10 μg/ml casein than with 100 μg/ml when F was present in the earlier stages of remineralization, with both groups achieving almost complete remineralization after 28 days.ConclusionCasein is a potential salivary phosphoprotein homologue that could be employed for in vitro dentine remineralization studies. Concentration related effects may be clinically significant and thus must be further examined. |
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| Keywords: | Salivary phosphoprotein Casein Remineralization Dentine |
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