Comparative in vitro investigation of the cariogenic potential of bifidobacteria |
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| Institution: | 1. UNESP - Univ. Estadual Paulista, Araçatuba Dental School, Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193, 16015050, Araçatuba, SP, Brazil;2. UNESP - Univ. Estadual Paulista, Araçatuba Dental School, Department of Restorative Dentistry, Rua José Bonifácio, 1193, 16015050, Araçatuba, SP, Brazil;3. UFRGS - Federal University of Rio Grande do Sul, Faculty of Dentistry, Department of Preventive Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003, Porto Alegre, RS, Brazil;4. UFRGS - Federal University of Rio Grande do Sul, Faculty of Dentistry, Department of Conservative Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003, Porto Alegre, RS, Brazil;1. Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan;2. Graduate School of Frontier Biosciences, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan;3. ERATO, Japan Science and Technology Agency, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan;4. Earth-Life Science Institute, Tokyo Institute of Technology, Meguro, Tokyo 152-8550, Japan;1. Department of Physics and Astronomy, Botswana International University of Science and Technology, P/Bag 16, Palapye, Botswana;2. Department of Physics, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya;3. Department of Physics, University of Botswana, P/Bag UB0704, Gaborone, Botswana |
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| Abstract: | ObjectiveThis study aimed to assess the in vitro cariogenic potential of some Bifidobacterium species in comparison with caries-associated bacteria.DesignBifidobacterium lactis, Bifidobacterium longum, Bifidobacterium animalis, Bifidobacterium dentium, Lactobacillus acidophilus, Lactobacillus casei, Actinomyces israelii, Streptococcus sobrinus and Streptococcus mutans were tested for acidogenicity and aciduricity by measuring the pH of the cultures after growth in glucose and bacterial growth after exposure to acid solutions. Biofilm biomass was determined for each species either alone or associated with S. mutans or S. mutans/S. sobrinus. Enamel hardness was analyzed before and after 7-days biofilm formation using bacterial combinations.ResultsB. animalis and B. longum were the most acidogenic and aciduric strains, comparable to caries-associated bacteria, such as S. mutans and L. casei. All species had a significantly increased biofilm when combined either with S. mutans or with S. mutans/S. sobrinus. The greatest enamel surface loss was produced when B. longum or B. animalis were inoculated with S. mutans, similar to L. casei and S. sobrinus. All strains induced similar enamel demineralization when combined with S. mutans/S. sobrinus, except by B. lactis.ConclusionThe ability to produce acidic environments and to enhance biofilm formation leading to increased demineralization may mean that Bifidobacterium species, especially B. animalis and B. longum, are potentially cariogenic. |
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| Keywords: | Biofilms Dental caries |
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