干扰素、阿糖胞苷单独及联合应用对慢性粒细胞性白血病细胞生长的影响

目的 探讨临床治疗慢性粒细胞性白血病（CML）更有效的方法。方法 以半固体集落培养，药物作用24小时液体培养及常规染色分析法研究干扰素（IFN）与阿糖胞苷（Ara-c)对K562细胞株和CML患者及正常人的骨髓单核细胞的生长影响。结果 IFN对K562细胞无明显抑制作用。IFN与Ara-c对CMLCFU-GM有优势抑制作用。对Ph^ 细胞存在选择性杀伤作用，IFN与Ara-c联合使用能增强IFN或Ara-c单药对CML白血病细胞的杀伤作用。结论 IFN与Ara-c可作为CML慢性期治疗的药物治疗，IFN与Ara-c联合治疗CML有望获得比单用Ara-c更好的治疗效果。

The effect of interferon alone, cytosine arabinoside alone and their combined effect on hematopoietic progenitors of chronic myeloid leukemia in vitro]

OBJECTIVE: In order to search for more effective treatment regimen of chronic myeloid leukemia (CML). METHODS: Semisolid culture and conventional chromosome assay were used to evaluate the effect of interferon (IFN) and Ara-c on K562 cell line and BMMNCs from CML patients and normal controls. RESULTS: IFN had no obvious inhibitory effect on K562 cell. IFN and Ara-c had preferential inhibitory effect on CFU-GM growth of CML and selective cytotoxic effect on CML Ph+ cell. The combination of IFN with Ara-c enhanced the cytotoxic effect of IFN or Ara-c alone on CML cells. CONCLUSION: IFN and Ara-c can be selected for therapy of CML in chronic phase. The combination of IFN with Ara-c may be more effective than IFN or Ara-c alone for the therapy of CML.