Two-photon laser scanning fluorescence microscopy using photonic crystal fiber |
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| Authors: | McConnell Gail Riis Erling |
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| Affiliation: | University of Strathclyde, Centre for Biophotonics, SIBS, 27 Taylor Street, Glasgow G4 0NR, Scotland. g.mcconnell@strath.ac.uk |
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| Abstract: | We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiation of less than 35 fs duration reaching the sample. |
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