Successful pregnancy and delivery from frozen-thawed embryos after intracytoplasmic sperm injection using round-headed spermatozoa and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome |
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Authors: | Kim S T Cha Y B Park J M Gye M C |
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Affiliation: | PL Infertility Clinic, 952-10 Dogok-Dong, Kangnam-Ku, Seoul 135-270, Seoul, South Korea. stkim@kyuh.co.kr |
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Abstract: | OBJECTIVE: To describe a successful pregnancy and delivery from frozen-thawed embryos after intracytoplasmic sperm injection (ICSI) and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome. DESIGN: Controlled clinical study. SETTING: IVF Laboratory, PL Infertility Clinic, Seoul, Korea. PATIENT(S): A couple with infertility resulting from globozoospermia with mosaic Down syndrome: 47,XY,+21[7]/46,XY[33]. INTERVENTION(S): Semen analysis, karyotyping, ICSI, assisted oocyte activation, assisted hatching, and frozen-thawed ET. MAIN OUTCOME MEASURE(S): Fertilization rate, implantation, pregnancy, and delivery. RESULT(S): Thirty-eight oocytes were aspirated, and round-headed spermatozoa were injected into 35 oocytes in metaphase II. Assisted oocyte activation with calcium ionophore A23187 after ICSI resulted in a high fertilization rate (21 of 35, 60%; 2 pronuclei in 18 of 21; 3 pronuclei in 3 of 21) and good embryo development. At 3 days after ICSI, 5 embryos of good quality were surgically transferred to the endometrium after assisted hatching, but no pregnancy occurred. After 2 months, the surgical transfer of 4 frozen-thawed embryos after assisted hatching led to an ongoing pregnancy. A female infant weighing 3,000 g was delivered at 38 weeks of gestation by cesarean section. CONCLUSION(S): We report the first successful pregnancy and delivery from frozen-thawed embryos after ICSI and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome. |
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