固相萃取-高效液相色谱法测定水源水中16种多环芳烃

目的:建立水中痕量16种美国环保署纳入"优先监测列表"的多环芳烃同时测定的方法。方法:水样中待测组分采用OASIS固相萃取柱富集,二氯甲烷洗脱,吹氮浓缩至近干并置换溶剂为乙腈,高效液相色谱分离,二极管阵列检测器与荧光检测器串联检测。结果:以乙腈-水为梯度流动相,PAH C18分离柱能有效分离16种多环芳烃组分,定量线性关系良好,加标回收率53.60%~91.05%,变异系数5.81%~10.77%,15种组分的最低检出限为0.15~5.38 ng/L,苊烯为33.37 ng/L。结论:本法具有非常高的选择性、灵敏度和准确度,完全能满足水中痕量多环芳烃的高灵敏分析。

Determination of 16 polycyclic aromatic hydrocarbons in sources of drinking water by solid-phase extraction with high-performance liquid chromatography

Objective:To develop a method for the determination of 16 priority environmental protection agency-defined polycyclic aromatic hydrocarbons(PAHs) in sources of drinking water by solid-phase extraction coupled with high-performance liquid chromatography(HPLC).Methods:The PAHs in sources of drinking water were concentrated on Waters Osasis HLB solid-phase column and eluted with methylene chloride,evaporated under nitrogen gas to near dryness and then reconstituted in acetonitrile for HPLC-fluorescence detection-UV techniques(HPLC-FLD-UV).Results:All 16 PAHs components were separated completely from each other on a PAH C18 column with acetonitrile-pure water gradient elution at flow rate of 1.20 ml/min,simultaneously quantified with good correlation coefficient of the standard curves.The average recoveries of 16 PAHs using spiked water sample ranged from 53.60%~91.05% and their relative standard deviation varied between 5.81% and 10.77%.The detection limits for 15 PAHs were from 0.15~5.38 ng/L,except for acenaphtylene(ACY) being 33.37 ng/L.Conclusion:The method developed is selective,sensitive,accurate,completely suitable for determination of trace PAHs analysis in sources of drinking water.