| 鼻NK/T细胞淋巴瘤的免疫表型、EBV感染及TCRγ基因重排的检测 |
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| 引用本文： | 刘繁荣,钟清玲,熊小亮,朱静,宋恩霖,温文.鼻NK/T细胞淋巴瘤的免疫表型、EBV感染及TCRγ基因重排的检测[J].肿瘤防治研究,2007,34(9):693-695. |
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| 作者姓名： | 刘繁荣 钟清玲 熊小亮 朱静 宋恩霖 温文 |
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| 作者单位： | 1. 南昌大学医学院病理教研室,330006
2. 深圳市第二人民医院病理科 |
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| 摘 要： | 目的 检测鼻NK/T细胞淋巴瘤（NK/TCL）的免疫表型、EBV感染及TCRγ基因重排,为诊断和鉴别诊断提供依据。方法 收集诊断鼻NK/TCL48例患者石蜡包埋标本,用免疫组化SP法标记LCA、CD79α、CD20、CD56、CD3、CD45RO及EBV抗体研究其免疫表型;EBER探针原位杂交方法检测EBV编码的小分子RNA（EBER）;聚合酶链式反应扩增方法检测TCRγ基因重排。结果 48例鼻NK/TCL均表达LCA,CD3、CD45RO、CD56和EBV阳性率分别为44%、52%、73%和19%,CD79α和CD20均阴性;EBER阳性率为81%;TCRγ基因重排阳性率为19%。结论 鼻NK/TCL免疫表型不一致,并非所有病例CD56阳性,石蜡切片中CD3阳性定位于细胞质;EBER在肿瘤细胞中高表达,提示它们可能为NK细胞来源;部分TCRγ基因重排阳性病例应为鼻NK样T细胞淋巴瘤。
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| 关 键 词： | 鼻NK/T细胞淋巴瘤 免疫组化 原位杂交 聚合酶链式反应 |
| 文章编号： | 1000-8578（2007）09-0693-03 |
| 收稿时间： | 2006-8-28 |
| 修稿时间： | 2006-08-282007-01-26 |
Immunophenotype and Epstein-Barr Virus Infection and Detection Rearrangement of T Cell Receptor γ(TCRγ) Gene in Nasal NK/T-cell Lymphoma |
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| LIU Fan-rong,ZHONG Qing-ling,XIONG Xiao-liang,ZHU Jing,SONG Enlin,WEN Wen.Immunophenotype and Epstein-Barr Virus Infection and Detection Rearrangement of T Cell Receptor γ(TCRγ) Gene in Nasal NK/T-cell Lymphoma[J].Cancer Research on Prevention and Treatment,2007,34(9):693-695. |
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| Authors: | LIU Fan-rong ZHONG Qing-ling XIONG Xiao-liang ZHU Jing SONG Enlin WEN Wen |
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| Institution: | 1. Department of Pathology , Medical College of Nanchang University , Nanchang 330006 , China; 2.Department of Pathology , The Second People’s Hospi tal of Shenzhen |
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| Abstract: | Objective To explore the immunophenotype and Epstein-Barr Virus(EBV) infection of nasal NK/T cell lymphoma and the significance of detection rearrangement of TCR γ gene in diagnosis and differential diagnosis of nasal NK/T cell lymphoma. Methods Fouty-eight cases of nasal NK/T cell lymphoma were studied, immunophenotype was analyzed by immunohistochemical staining for LCA、CD79α、CD20、CD56、CD3、CD45RO and EBV with SP method. In situ hybridization (ISH) with EBER1/2RNA probes was perfomed.TCR γ gene rearrangeme... |
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| Keywords: | NK/T cell lymphoma Immuneohistochemistry In situ hybridization Polymerase Chain Reaction |
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