用HRP法探讨胃各部的交感传入神经元的节段性分布

<正> 神经解剖学家Peele认为胃和肠的感觉纤维位于迷走神经和交感神经中,但痛觉冲动主要由交感神经传导,恶心感觉则由迷走神经传递,饱满感和胃充实感亦然。Ca-rpenter叙述了内脏有许多传入纤维(包括有髓鞘和无髓鞘两种),内脏痛从腹部至盆腔脏器主要是通过交感神经传入的,迷走神经感觉纤维则关系到内脏运动和分泌反射等。此外,Ranson和Billingsley等神经解剖学家对内脏感觉纤维的论述和Peele及Carpenter等是一致的。至于交感感觉纤维的胞体位于脊神经节的问题已为诸学者所公认。从临床实践证明内脏的痛觉及

APPLICATION OF THE HRP METHOD TO EXPLORE THE SEGMENTAL DISTRIBUTION OF THE SYMPATHETIC AFFERENT NEURONS OF THE STOMACH (ANTERIOR WALL,POSTERIOR WALL,PYLORUS AND CARDIA)

The posterior wall of stomach in 9 rabbits, the anterior wall of sto- mach in 6 rabbits, the pylorus in 9 rabbits and the cardia in 10 rabbits were used. In each animal 80～100 ul of 10% horseradish peroxidase (HRP, sigma Ⅵ) solution was injected into the tunica serosa of the stomach. After survival of five days, the animals were sacrificed and their he- arts were perfused with 2% paraformaldehyde soluted in 0.1 M phosphate acid buffer (pH 7.2～7.3). The spinal ganglia were treated according to HRP method and incubated in 3-3 diaminobenzidine (DAB) H_2O_2 solution. The distribution of the HRP labeled cells were examined under light micro- scope and results were as follow: After injection into the tunica serosa of anterior and posterior wall of stomach, we could find clearly labeled cells appearing in the total thoracic spinal ganglia (T 1～12) as well as cervical spinal ganglia four to eight (C 4～8), and lumbar spinal ganglia one to seven (L 1～7). Thoracic spinal ganglia T 5～9 were the segments where the labeled cells were most nume- rous, less in the cervical ganglia, and least in the lumbar ganglia. After the HRP was injected into the pyloric region, we could find la- beled cells appearing in the thoracic spinal ganglia one to twelve (T 1～12) and lumbar spinal ganglia one to four (L 1～4). When the HRP was injected into the cardiac region, labeled cells could be found cervical spinal ganglia 4～8 (C 4～8) and thoracic spinal ganglia 1～12 (T 1～12), but in the least number. Moreover, it was found that the labeled cells were of different sizes and that labeled cells were mainly small cells, with comparatively less large cells. The above experimental study further suggested that the segment of the sympathetic afferent fibers of the stomach was longer.It also provided a morphological foundation for explanation of the theory of the somato- viscera interrelated connections.