| HPLC法测定蒙药那如-3丸中胡椒碱的含量 |
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| 引用本文： | 陶淑娟,王桂燕,毕开顺. HPLC法测定蒙药那如-3丸中胡椒碱的含量[J]. 沈阳药科大学学报, 2004, 21(1): 35-37 |
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| 作者姓名： | 陶淑娟 王桂燕 毕开顺 |
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| 作者单位： | 1. 沈阳药科大学制药工程学院,辽宁,沈阳,110016
2. 沈阳药科大学药学院,辽宁,沈阳,110016 |
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| 摘 要： | 目的建立蒙药那如 3中胡椒碱的含量测定方法。方法反相高效液相色谱法。采用Hyper silC18色谱柱 ;以甲醇 水 (V∶V =77∶2 3 )为流动相 ;流速为 1 0mL·min-1;柱温为室温 ;检测波长为3 43nm ;进样 2 0 μL;外标法计算含量。结果胡椒碱在 1 2～ 84mg·L-1内峰面积与对照品质量浓度呈良好线性关系 ,其回归方程为A =1 64× 1 0 5ρ -9 9× 1 0 3 ,r =0 9998,其平均回收率为1 0 1 4% ,RSD为 1 4% (n=9)。结论方法简便、准确 ,为那如 3的质量控制提供了方法。
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| 关 键 词： | 那如-3 胡椒碱 反相高效液相色谱法 |
| 文章编号： | 1006-2858(2004)01-0035-03 |
| 修稿时间： | 2003-01-27 |
Determination of the content of piperine in the Mongolian recipe Naru-3 by HPLC |
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| TAO Shu-juan ,WANG Gui-yan ,BI Kai-shun. Determination of the content of piperine in the Mongolian recipe Naru-3 by HPLC[J]. Journal of Shenyang Pharmaceutical University, 2004, 21(1): 35-37 |
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| Authors: | TAO Shu-juan WANG Gui-yan BI Kai-shun |
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| Affiliation: | TAO Shu-juan 1,WANG Gui-yan 1,BI Kai-shun 2 |
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| Abstract: | Objective To determine the content of piperine in the Mongolian recipe Naru-3. Method The content of piperine in Naru-3 was determined by RP-HPLC with Hypersil C_ 18 column at room temperature.The mobile phase was methanol-water ( V∶V =77∶23) with a flow rate of 1.0 mL·min -1 and the injecting volume was 20 μL .The external standard was used and the wavelength of the detector was set at 343 nm .Results The linear range was 12～ 84 mg·L -1 ,and correlation coefficient was 0.999 8 .The average recovery and the relative standard deviation were 101.4% and 1.4%,respectively.Conclusion The method is simple,accurate,and can be used for the quality control of Naru-3. |
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| Keywords: | Naru-3 piperine RP-HPLC |
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