The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus |
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| Authors: | M M Binns M E Boursnell I J Foulds T D Brown |
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| Affiliation: | Houghton Poultry Research Station, Houghton, Huntingdon, Cambs PE17 2DA, U.K. |
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| Abstract: | An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences. |
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| Keywords: | cDNA cloning coronavirus IBV oligonucleotide primers |
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