随机引物PCR方法用于霍乱分子流行病学研究

研究建立了随机引物PCR方法用于霍乱分子流行病学研究,其中两组引物针对霍乱弧菌重复插入序列设计,一组引物为任意序列.对2株霍乱弧菌O1群古典型(CVC)、81株埃尔托型(EVC)和10株O139群进行了分析,上述菌株经PCR鉴定,均携带霍乱肠毒素(ctx)基因和毒力协同调节菌毛(tcp)基因.2株CVC分为2个类型,81株EVC分为14个类型,10株O139群分为3个类型.其中10株EVC分离于一次霍乱暴发,分为2个类型,提示该次暴发可能存在多个传染源.随机引物PCR方法简便、快速、分辨力高,在霍乱分子流行病学研究中有较大的应用前景.

Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Epidemiological Studies of Vibrio Cholerae

Random amplified polymorphic DNA(RAPD) was established and was applied in molecular epiemiological studies of Vibrio cholerae.Two groups of primer sets were aimed at repetitive sequence of Vibrio cholerae,one group of primer was arbitrary sequence.2 O1 classical(CVC),81 EI Tor and 10 O139 strains were analysed wiht this mehtod.All strains have cholerae toxin gene and toxin coregulated pili gene with the detection of PCR.2 CVC,81 EVC and 10 O139 strains were divided into 2,14 and 3 RAPD types respectively.10 EVC strains isolated from one explosive event were divied into 2 RAPD types.It means that there were several infectious origins in this event.RAPD provides a rapid,simple and discriminable method in molecular epidemiological studies of Vibrio cholerae.