多环芳烃致4种细胞彗星尾矩和双核细胞微核率变化差异的研究

目的探讨彗星尾矩和双核细胞微核(CBMN)率两个指标对多环芳烃反应动力学的差异。方法应用4株细胞系,分别是中国仓鼠卵巢细胞CHO-K1、小鼠成纤维细胞NIH3T3、中国仓鼠肺成纤维细胞CHL、人胚肺成纤维细胞HELF,给予不同浓度的苯并(a)芘[B(a)P]处理,观察彗星尾矩和CBMN率的变化情况。阳性对照分别是过氧化氢和环磷酰胺。结果在所应用的B(a)P浓度范围内,4种细胞均在较低剂量下就出现了明显的拖尾(CHO-K1:0.5μmol/L;NIH3T3:0.75μmol/L;CHL:0.5μmol/L;HELF:0.3125μmol/L),并且随作用浓度的增加,彗星尾矩呈直线形上升趋势,各组与其邻近的较低剂量组相比,差异有显著性。CBMN率在中剂量组才出现显著升高(CHO-K1:2.0μmol/L;NIH3T3:1.5μmol/L;CHL:2.0μmol/L;HELF:1.25μmol/L),达到一定作用剂量后,CBMN率的上升趋于平缓,其剂量反应曲线基本呈S型。结论彗星尾矩和CBMN率对多环芳烃动力学反应模式不同,敏感性不同,在进行环境致癌物早期效应标志物研究时,应合理地将两种方法相结合,综合评价化学物所致遗传损伤。

Different change of olive tail moment and cytokinesis-biock micronucleus frequency of the cells of 4 kinds treated with polycyclic aromatic hydrocarbon

OBJECTIVE: To investigate the difference change of olive tail moment (TM) and cytokinesis-block micronucleus (CBMN) frequency of the cells of four kinds treated with polycyclic aromatic hydrocarbon. METHODS: Four different cell lines, CHO-K1, NIH3T3, CHL, and HELF were treated with B(a)P in vitro, and the genetic damage were detected by Comet and CBMN assay, respectively. The positive controls were Hydro peroxides (H2O2 ) and Cyclohosphamide (CP) for Comet and CBMN assay, respectively. RESULTS: The changes of olive TM levels of four cell lines treated with B(a)P were occurred at the lower doses of B(a)P (CHO - K1:0.5 micromol/L, NIH3T3 : 0.75 micromol/L, CHL : 0.5 micromol/L, HELF : 0.3125 micromol/L) than that of CBMN frequencies (CHO-K1 : 2.0 micromol/L, NIH3T3 : 1.51 micromol/L, CHL : 2.0 micromol/L, HELF : 1.25 micromol/L). The dose-response curve of B(a)P was linear for Olive TM, but sigmoid for CBMN frequency. CONCLUSION: The data showed that olive TM was more sensitive than CBMN frequency for the genotoxic detects of B(a)P for the four cell lines.